LOCUS       sequence-229162-        9840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_162520-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5004..5021
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5073..5128
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5127..5146)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7364..7382)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7450..7554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7555..7623,7624..8415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7773..7792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8586..9174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9075..9094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9328..9345
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9462..9483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9498..9503,9504..9521,9522..9528)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9536..9552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9541..9563
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9560..9576
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9560..9576
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 atagcggcgt ggtggacgtg atggaagatg ccagacccgg caccatcatc atcgacaacc
     1261 tggaacaccg gttccccatc ctgaacagcg gcgagttcaa cagccactac ttcgccatcg
     1321 gcaaccccag cagccccggc atcaaccacc tggaaatccg gcggcacaga ttcgacaaca
     1381 gcgtgcagct ggtggtcaag gacggccaga acggccccga ccgcgaggaa ctgtgtggcg
     1441 ccagagagac acagagcgac agccagatgc ccttctgcga catcaccctg atcatcctgc
     1501 acggcaagcg gcaggaaccc agctacggca agctgaccct gcggatcctg gaccggaacg
     1561 acaacagccc catcttcacc aagcgggaca ccaccgagct gcggatcccc gagaacatcg
     1621 agagcgtgga catgctgggc agcagcgcca ccagccacag ccagatcctg cacagcaaca
     1681 acaagcccac cagcggcaac cctaccaccc tggaactgcc caaggcccac gacatcgacc
     1741 tgcccgagaa cggcgtgaaa acctaccggc tgaccaccgt gtccggccac gagatcgaca
     1801 aggccctgtt caacctgatc gtgaacaacg acaacgagaa caagatcggc aacagcctgc
     1861 ccgtgccctt cctgcggatc gtgggcctgc tggacagaga gaagcaggac gagtactggt
     1921 tccatctgct ggccatcgac ggcggcaatc ccaagagaac cggcacccag accatccacc
     1981 tggtggtcac cgacatcaac gacaacatcc ctaagttccg gaagcccatc ttccacttcc
     2041 ccaccttcat caccaccagc gacctgtacg gcaacggcgc cacccaggaa tttctgaagg
     2101 tccccgagac acagaccccc ggcaccccca tcgtgaccat cattgccgac gaccccgaca
     2161 aggacctgaa cggccagatc acctacagac tgaaagagag cctgaacggc gaccagaacg
     2221 ccatcagcat gcagtggttc gacctggaaa caaacaacgg caccgccatc ctgaaggtgg
     2281 ccaagaagat ggacgccgac gaccggcacg gcgcctcttg gagcgacctg aacagagagc
     2341 acagcggcag actgatcaag ctggtggtgg aagccgtgga cgccggcagc cctagcctga
     2401 caggcagcat cgagctgctg atcctggtgg aaaacatcaa cgataacgag cccgtgatca
     2461 gcgtgcagta cacccagccc ttccagaccg agagcgagtt ccacaactcc gtgaagggca
     2521 agatcgtggg ctgcctgcag gaaaaccaga acgagcccct gacaatcgcc cacctgaccg
     2581 tggaagatgg cgacatcatc gagagcaact ccggcgccat ctccaacctg ctggaaatcc
     2641 actgcgagac aaacgatacc cggttcctgc tcgagaaggt gtccaacctg aactacgccg
     2701 acagaagcga cagctacggc ctgagcacca cccacttcga cagccccctg ctgttctaca
     2761 agatgagcag cctgaagtcc atcgaccggg aagccgagcc ctgggtgttc gtgaaagtga
     2821 tctgcgtgga ccaggtccag gtgtcctacg ccctgtccgg ctaccagaac atcggaaagc
     2881 tgatccagag cagccggctg accggcagca ccacagtgtc catcaaggtg ctggacgtga
     2941 acgataatat ccccaagttc gcctccggca actaccagtt cagcgtgttc gagacaccct
     3001 ctatcgccgt gaacctgatc aagaacatgt tcgacgacca gagcaccgtg gaaatcggcc
     3061 gggtgtccgt gaacgaccag gacgagggcg tgaacagcaa ggtgtcctat cggttcctga
     3121 gcggcgagat cgacgccttc aagatcgacg agaacaccgg cgtgatcaga agagtgggct
     3181 tcatcgaccg cgagaaagtg aaccagatgg aactggtggt cgaggccaag gaccacggcg
     3241 agcctagcct gagcagcacc accgtgatca aagtggacgt gctggatgtg aatgacaacc
     3301 ccccctactg gatcatgagc agccccaacg accgggacaa agagatccgg cggagcggcc
     3361 ctgaggacgg cgtgtactac ttcagcctga acgaggacgc ccccatcggc agcatcgtgg
     3421 gcaccatcag cgccgtggac accgacggaa tcgccgagtc cgagatgatc gagcagatca
     3481 tcgagaagca gaaccccccc aagctgcaga aaacaaactt cggcaatctg ctgggcacca
     3541 gcagcatcac ctaccagctg gaaaacgagg gcgacggcaa gatcttcagc atcaacagca
     3601 gaaacggcga catccggctg aacaagcacc tggatagaga gatccgggcc cactacgagt
     3661 tccgggcctt tgccatcgac gacatcctga agtccgtgaa aaccagccag aacaacccct
     3721 acctgatcaa caagtggcag caccagtaca ccgccaccgc cacaatcatc atcacagtgc
     3781 tcgatgtcaa cgacaatccc cctatcttcg aaacccccct gaatggccag gaattccaca
     3841 tcgagcccgg cagcagcatg accaccgccg gcagcacact gttcaccgcc aaggcccatg
     3901 accccgacat cggcgacaat agcctcgtgc ggtacagcct ggacaacaac ggctacggca
     3961 tcgtggaaat tgactccacc accggcgtgt gttacttccg ggaaaccctg cagtacagcc
     4021 tgatgaacaa gctgatcagc agcaatcagt acgccatcaa cggccggacc agcaatcacc
     4081 tgaccgacag caacctgatc accagcgaca atctgatcac caacagagcc cacgagctgc
     4141 acagcttcag cctgtccctg accattatcg cccgggacct gggcaccccc tactccctga
     4201 acaacacccg gaccgtgaag ctcgtgtgga ccaccgagag ccagatgaag tccctgagca
     4261 tcagcaacaa cgacctgatc agctccagca tcttcggcaa cttcgagttc ctgtccgaca
     4321 accggatgac catcaacaag ctcatcatcc ctggcgcgcc gtcgacctcc atcacggcct
     4381 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     4441 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     4501 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     4561 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     4621 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     4681 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     4741 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     4801 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     4861 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     4921 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     4981 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     5041 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     5101 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5161 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     5221 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     5281 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     5341 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     5401 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     5461 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     5521 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     5581 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     5641 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     5701 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     5761 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     5821 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     5881 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     5941 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     6001 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     6061 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     6121 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     6181 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     6241 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     6301 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     6361 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     6421 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     6481 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     6541 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     6601 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     6661 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     6721 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     6781 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     6841 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     6901 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     6961 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     7021 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     7081 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     7141 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     7201 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     7261 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     7321 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     7381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     7801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     8101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8701 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9361 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9421 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9481 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9541 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     9601 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     9661 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     9721 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     9781 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
