LOCUS       sequence-229164-        7677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_166300-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2841..2858
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2910..2965
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2964..2983)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5201..5219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5287..5391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5392..5460,5461..6252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5610..5629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6423..7011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6912..6931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7165..7182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7299..7320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7335..7340,7341..7358,7359..7365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7373..7389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7378..7400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7397..7413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7397..7413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 accacgagaa gtgcttttgc tacctgaaca gcaccaccaa cctgccctac cacaccacca
     1261 acaacaccaa cgtgaagcag ctgatccact gcagcctgac ccagctgagc tacaaccaca
     1321 gcgaggaact gagctgccag cgggtggaca agttcatctg catcggcccc tacctgagca
     1381 acgacagcca catcaacctg agcatcaacc cctgggcctt cagcaacctg ttcgtgtcca
     1441 acagcgtgaa caccctgcag ctggacttca acatcaacac ccaccagctg aacgccctga
     1501 ccctggccgg catggacggc ctgctgaacc tgctggccag aaccagcctg ctgagctggg
     1561 acagctgcag cttcatcggc ctgcccaacc tgcacgagat caccctgaac tgcaaggccg
     1621 tgttccccga gttcctgacc ttcggctcct tcatcaccct gatcagattc gtggactgcg
     1681 agggcgtgcc cctgcagttc tactgcatcc agtgtatcca gaaccccaac gtgtacgtga
     1741 tcagaatcag acccggccca cccctgcggc agtacctggt caagttcacc cggctgagca
     1801 gcagcagcgg cagctccacc agcagcctga acaacaagat catcgagaac aacgagaacc
     1861 tgagccccaa ccaccatctg cccctggaca gctgcgtgta cggcgtgtgc agcgacgaga
     1921 tgctgtgccg ggacaacctg cccctgaaac aggtgcaggc cggcatcggc cagcccgaga
     1981 agcccatcat cgagatcgtg tccaatgacg agatcctgat ccctaacacc accagcatgg
     2041 acctgtacga cagcatgcag ctgaccagca tccagcccat cctgaacacc acccccagca
     2101 tcatcgagcc catcaacacc acctccgtgt acgacatcgt gatcctgacc aaatacggcc
     2161 acagcaagac cgaccaccgg cagaagattg gcgcgccgtc gacctccatc acggcctata
     2221 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2281 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2341 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     2401 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     2461 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     2521 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     2581 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     2641 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     2701 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     2761 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     2821 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     2881 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     2941 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3001 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     3061 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     3121 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3181 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3241 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3301 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3361 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     3421 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     3481 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     3541 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     3601 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     3661 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     3721 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     3781 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     3841 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     3901 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     3961 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4021 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     4081 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     4141 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4201 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4261 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4321 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4381 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     4441 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     4501 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     4561 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4621 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4681 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4741 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4801 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     4861 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     4921 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     4981 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     5041 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5101 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     5161 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5221 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5401 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5461 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5521 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5581 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5641 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5701 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5761 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5821 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5881 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     5941 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6001 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6061 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6121 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6181 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6241 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6301 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7081 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7141 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7201 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7261 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7321 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7381 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     7441 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     7501 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     7561 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     7621 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
