LOCUS       sequence-229168-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_157070-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2988..3005
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3057..3112
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3111..3130)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5348..5366)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5434..5538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5539..5607,5608..6399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5757..5776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6570..7158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7059..7078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7312..7329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7446..7467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7482..7487,7488..7505,7506..7512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7520..7536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7525..7547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7544..7560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7544..7560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tctaccccgg cgacgactgc aacaagtgcc ggcacgccgc caagagcttc atcctgggct
     1261 tccacaagag cgccggcaag aacttcggcg gaggcaacag cctgtgggag gaaaagcacc
     1321 tgggcagcta cagcgtgtcc gaagtgcgct accacgacat cgtggaaggc atctgcagcg
     1381 acgtgaagca ggccgtgaag tgccacgagt tcatggaatc catcgagcac cacctggaag
     1441 attggtggct gcgggactac agaagctacg ccaacaagat cgagggcttc gaagaggacc
     1501 tgtgcgtgac ccggaccaag ttctgctgcc ccagcaacag cttcggcaac agctgcctgc
     1561 cctgcccccc ctgctacttc agcggcggca gatgcgacgg caacggcacc agaagcggca
     1621 ccggcagctg catctgcaac gatggctaca ccggcgagct gtgcgacaag tgcaccttcg
     1681 agacacactt ccagagccac aacgacagca gcatcagctg cctgagatgc cacctgagct
     1741 gcagcggcgg ctgtagcggc ccctaccctg agaattgcag cgcctgtgcc agcggctgga
     1801 ccgagatgga ctctggcgat cagcggggct gcgtggacat caacgagtgc gtggacagcc
     1861 cctgcaacag aagcacccac ttttgcctga acaagcccgg cagctacgag tgtgtgccct
     1921 gccacagcgc ctgtaacaag tgcagcggcc ccaccgccaa cgactgcgag agctgtgcct
     1981 ctggctacca gaggggcaac ggcgacgtgt gcgaggatat caatgagtgc aacgccgaca
     2041 gcaacatctg caacagcgag ggggagctgt gcaccaacac cgtgggcagc ttcaagtgcg
     2101 actgcaagag cggctacaag cggaccagca gcggctgcgt gctggccgtg aagcccgaga
     2161 agggcagcaa gaccagcgac aagttcgaga caaagtccac cgtgcacaac atcaacagcc
     2221 ggaaccggaa cacccagcgg atcatctgga ccgtgaccta caccaaagag tttctgaagt
     2281 acagcgccag cctggccgcc ttcggcacct tctgttggtt cgccaagggc caccctggcg
     2341 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2401 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2461 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2521 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2581 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2641 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2701 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2761 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2821 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2881 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     2941 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     3001 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3061 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3121 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     3181 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     3241 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3301 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3361 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3421 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3481 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3541 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3601 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3661 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3721 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3781 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3841 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3901 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     3961 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4021 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4081 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     4141 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     4201 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     4261 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4321 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4381 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4441 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4501 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4561 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4621 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4681 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4741 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4801 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4861 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4921 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     4981 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5041 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     5101 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5161 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5221 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5281 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5341 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5401 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5461 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5521 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5581 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5641 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5701 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5761 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5821 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5881 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5941 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6001 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6061 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6121 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6181 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6241 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6301 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6361 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6421 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6481 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7261 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7321 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7381 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7441 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7501 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7561 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7621 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     7681 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7741 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7801 ttggctcatg tccaatatga ccgc
//
