LOCUS       sequence-229170-        8634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_136690-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3798..3815
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3867..3922
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3921..3940)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6158..6176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6244..6348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6349..6417,6418..7209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6567..6586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7380..7968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7869..7888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8122..8139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8256..8277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8292..8297,8298..8315,8316..8322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8330..8346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8335..8357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8354..8370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8354..8370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 acaccctgaa gcccatcgag atccctctga cccacggcgg cagcgtgatc ggcaaagagg
     1261 aaatcgtcaa catcgacggc caggaagtga aagtcaacag cttcctgggc atcccctacg
     1321 ccagcaagcc catcggcaag ctgagattcg cccctcccga gaagcacccc ggctggaagg
     1381 gcaagtacaa cgccaccacc ctgtccccca cctgttggca gtacatcttc accggcttcg
     1441 acgccgtgaa cgccgctggc aagatgtgga tcaacaacac cgagatgagc gaggactgcc
     1501 tgtacctgaa cgtgtggacc cccaagagca gcgtggacag cccccatctg cccgtgatgg
     1561 tctggatcta cggcggaggc ttcaccagcg gcagcgccaa cctgcaggtc tacaacggcg
     1621 ccatcctgag cgccacccag aacgtgatca tcgtgtccat gcagtacaga gtgggcgcct
     1681 tcggcttcct gcggctgaag cccaacatca ccgaccagac ccagaccgac gccctgggca
     1741 accagggcct gctggatcag ctgatggccc tgaaatgggt gtccgagaac atcggccagt
     1801 tccacggcga ccccaaccag gtcacaatct tcggcgagag cgctggcgcc gtgtccgtgt
     1861 ccatcctgtg gatgagccct atcgcccagc cctacttcag acgggccatc ctgcagagcg
     1921 gcagcctgta cgccagatgg ggcctggaca acgccgacga ggcccacgag aaggccgacg
     1981 tgttcaccag agagtgcggc tgccagagcc ccagcgtgga ccggaaggcc agcctggaat
     2041 gcctgcggaa gctggacccc ctgaccctgg tcaaccagct ggacagcatc aacgtggcca
     2101 tcggaaagca cagatacgac gccgtgcgga agtacctgct gcccagatac cacaagcagg
     2161 aaccctttct gctgagccag agcaccagca cccggctgta cttcgacgtg cccctgcagc
     2221 ccgtgatcga cggctacctg gtgcccaagc accccgacca catcttcaac gagaagaaca
     2281 agctgaagca gaaccccgag ctgctgatcg gcgtgaacac caacgaggcc atgttctttc
     2341 tgctgcccgg aatcgctatc aaggataccc agttcctgtt cagcaacggc tccgtgatca
     2401 tgcccagcac catggaactg gctggcaaga agaagccctt caaagagggc gaggaaatcg
     2461 ccgacttcta ctggatcacc gccacacaga tcctggacga gagccacatg cggcctggcc
     2521 tggccaagat gcccagctac tactacaacc tgcccctgac cagcagcccc aagcggggct
     2581 actacgaccc cgacaccgtg tacatccacg acgaggaact gctgcggcgg ctggacaagt
     2641 tcgccggcga cctggatttc gcctgcccca ccctgaactt cgccgaacag gtggcccggc
     2701 tgcccaacgc caaggtgttc ctgtaccact tcaacaagcg gaccgagagc ctgcccatgc
     2761 ccaagtggac aggcgtgatg cacggctacg agatcgagta tatcttcggc atcccttacg
     2821 accctgagtt cagcaagcag ttctacaact tcaccgaccc cgagaagatc ttcagcagcc
     2881 ggatcatgaa gatgtggacc aatttcgcca agaccggcca ccccagcaag agcaacgacg
     2941 gcaagatcag cacccccgag tggcccctgt tccacagcac cgacggcttc gtgtccaaca
     3001 accccgacta cctgatcctg gaagatgaga caaagctggg cagcggcctg caccgggaca
     3061 gatgcgcctt ctggctgcac gagatgcagg acatgaagga catctggttc aacagatgcg
     3121 accccagcgg cggcatcaag cccacaggcg cgccgtcgac ctccatcacg gcctataaga
     3181 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     3241 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     3301 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     3361 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     3421 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     3481 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     3541 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3601 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3661 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3721 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     3781 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     3841 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     3901 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     3961 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     4021 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     4081 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     4141 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     4201 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     4261 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     4321 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     4381 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     4441 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     4501 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4561 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4621 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4681 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4741 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4801 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     4861 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     4921 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     4981 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     5041 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     5101 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     5161 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     5221 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     5281 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     5341 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     5401 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     5461 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     5521 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5581 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5641 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5701 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5761 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     5821 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     5881 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5941 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     6001 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6061 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     6121 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6181 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6241 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7501 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8041 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8101 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8161 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8221 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8281 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8341 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     8401 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     8461 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     8521 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     8581 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
