LOCUS       sequence-229171-        8100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_061970-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3264..3281
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3333..3388
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3387..3406)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5624..5642)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5710..5814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5815..5883,5884..6675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6033..6052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6846..7434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7335..7354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7588..7605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7722..7743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7758..7763,7764..7781,7782..7788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7796..7812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7801..7823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7820..7836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7820..7836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 tcggcctgtt catcttcatg agcggcctga tgcccaacga ccccgccacc aacgtggaaa
     1261 agatcggcta cgtggaagag acatacaaga tcgacaagtt cgtgatgatc gtggtggacg
     1321 ccctgcgggc cgacttcctg ttcagcccca agtacagcga gaactgggtc aagctgcgga
     1381 gctacatgaa cctgcagagc gccacctgta gcgccagcat cgtgcagccc cctaccgtga
     1441 ccctgccccg gatcaaggcc atcgtgtccg gccggaaccc caagttcgtg gacatcctgc
     1501 acaacgtgaa cgccgagaca atggtggacg acaactgggt cacccggctg gtggaccacc
     1561 agtggaacat ccagttctac ggcgacgaca cctggatcaa gctgttcccc aagagcttcc
     1621 aggtgtacga gggcaccaac agcttctacg tgaacgactt ctacgaggtg gacgccaacg
     1681 tgacccggca catcagcacc ctgatgacca cccccgacaa gtgggactgc ctgatcctgc
     1741 actacctggg cctggaccac atcggccaca tcgagggccc tagcggcagc agcatcccca
     1801 agaaaatccg cgagatggac gaagtgatcc accggatcct ggaagtgctg atgaacagca
     1861 gctccatcgt gaacaagaac tggctgttca tcctgaccgg cgaccacggc atcagcgaca
     1921 agggcagcca cggcggcagc accaccggcg agaaaaccac cggcctgttt atgttcggca
     1981 gcaactggaa caagaccaac gaccacctga ccgagaatac ctgcgtgacc accaacctga
     2041 agcagctgcc ccagacccag caggtcgacc tcgccaccct gatcggcctg atcaccggcg
     2101 tgggaatccc tgtgcggagc ctgggcatcc tgagccccag ctggctgcac aacttctggt
     2161 ccaccaacca cgaccggctg aaggtgctgt acaccctgat ccagcacgtg ggccggctga
     2221 gcggcgagtg cgccttcagc ctgaagtcca accacgaact cgagtacaag aactgcatca
     2281 gcaacgtgtg gtccgaggaa cggaccacca agtacaacat cctgctgaac gagatcatca
     2341 cctggctgca ctgccagaag atccagggca actccaccac cgagttcaag cagatcgact
     2401 gcgtgaagtc cggcagcaga ctgaagggcg aggaactgat caccaacacc gtgaacctgc
     2461 tgaatggcat ccagagcgag agcctgagca aggtggaaca cctgagcaac aagcagatga
     2521 tcttcggcgg catcctgatg tggatcaaca agaagtctct gatccagtac tactacagcg
     2581 acaccgccga cctgagctac agcttcgacc ctggcgcgcc gtcgacctcc atcacggcct
     2641 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2701 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2761 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2821 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2881 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2941 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     3001 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     3061 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     3121 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     3181 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     3241 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     3301 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     3361 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3421 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     3481 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     3541 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3601 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3661 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3721 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     3781 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3841 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3901 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     3961 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     4021 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     4081 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     4141 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     4201 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     4261 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     4321 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     4381 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     4441 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     4501 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     4561 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4621 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4681 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4741 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     4801 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4861 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4921 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     4981 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     5041 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5101 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     5161 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5221 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     5281 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     5341 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     5401 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5461 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5521 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     5581 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5641 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5701 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5761 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5821 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5881 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5941 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6001 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6061 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6121 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6181 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6241 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6301 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6361 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6421 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6481 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6541 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6601 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6661 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6721 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6781 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6961 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7441 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7501 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7561 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7621 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7681 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7741 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7801 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7861 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7921 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     7981 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     8041 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
