LOCUS       sequence-229174-        7089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_064430-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2253..2270
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2322..2377
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2376..2395)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4613..4631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4699..4803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4804..4872,4873..5664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5022..5041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5835..6423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6324..6343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6577..6594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6711..6732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6747..6752,6753..6770,6771..6777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6785..6801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6790..6812
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6809..6825
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6809..6825
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctagacctct gacaccttac cagagaagct tcacccccgc caagaacatc agctgcgccc
     1261 agtgcgagat gaagctggaa gtggacaaga gcaagagctt cgtggaaacc gacatgtacc
     1321 ggcggagatg cctggacgag cccgagctgt tctacaagcc ctgcatcgtg aacgccaaga
     1381 atacccccag aggctgcgcc aagctgacca cctacctgaa agaacccgtg gccaagggca
     1441 gagtgaaaga agtgaccctg atgaagcggt tctgcgccac agagggcgcc gagcccgaag
     1501 agatcagatg cgtgaacaga gccagcgagg gcggctacag cgagctgtgt atctgcggca
     1561 ccgacaactg caacagcgcc cctcagctgc tgaagtcctc tggcgcgccg tcgacctcca
     1621 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     1681 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     1741 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     1801 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     1861 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     1921 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     1981 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     2041 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     2101 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     2161 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     2221 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     2281 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     2341 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2401 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     2461 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     2521 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     2581 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     2641 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     2701 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     2761 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     2821 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     2881 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     2941 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     3001 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     3061 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     3121 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     3181 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     3241 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     3301 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     3361 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     3421 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     3481 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     3541 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     3601 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     3661 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     3721 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     3781 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     3841 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     3901 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     3961 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     4021 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4081 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     4141 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4201 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     4261 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     4321 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     4381 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     4441 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     4501 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     4561 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     4621 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5041 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5341 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6541 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6601 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6721 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6781 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6841 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     6901 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     6961 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     7021 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     7081 tatgaccgc
//
