LOCUS       sequence-229178-        8568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_141680-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3732..3749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3801..3856
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3855..3874)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6092..6110)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6178..6282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6283..6351,6352..7143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6501..6520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7314..7902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7803..7822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8056..8073
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8190..8211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8226..8231,8232..8249,8250..8256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8264..8280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8269..8291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8288..8304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8288..8304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcaacgacaa caacctgtgg caggtcatca ccagctaccc caacgccacc aagttcgccg
     1261 acttcatcca ggaaatcggc ctgcagagca gcctggaaaa gcccggctgc ctgggcgacg
     1321 acgagtgcct gaccatcatc ctgcccacca acgacgccat cgacctgatg gccaacgacc
     1381 tggcctggta ctccctgggc gactaccaga agcacagctt catccacggc cacatcatcc
     1441 agcggggcat caccaccggc cagagcagcg tcagagtgac caccagagag tggaccaagc
     1501 tgggccagca gatgaacttc atcgacatcg gcttcggcac cggcgtgaag tacaccaccc
     1561 tgttcgccaa gcagagattc ggcttcagca caatcgccga cagcaccccc ctgtacttcg
     1621 tgaacaacgc caagatcatg acccccgaca tcatggccag caacggcatc atccagatcg
     1681 tgaaccgggt gctgtacacc ccccacatca gcctgccctt catggaattt ctgcagaagc
     1741 agggcaacct gggcatcagc aaagagctgt ggtatctgct gctgcaggaa cctaagtacg
     1801 ccctgtaccc cgcccagcag atctacagca ccatcttcgt ggtgtccgac agcggctgga
     1861 acagcctgcc catcacccag ctgaaccggc tgcggaagaa catcaccctg ctggccagcg
     1921 tgctgagcta ccactactgc cccaatcagc tgatctaccg cgagtggatc agcgtgaagc
     1981 ccaacatgaa catctacacc ggcttcccca accggcccca gagcgccagc tctatccagc
     2041 ccagcgagct gaacagcgcc cagctgtaca agagcagcgg cgacagcatc atcatcagca
     2101 gcggcaacac cgaggccaag ctggaagata agagcttcat cattcagaag gccatcgtcc
     2161 acattatcca gaagcccctg gccttcatct tcgagacacg ggaagagatc ctgaaccgga
     2221 tcaacagcaa cctgctgtac agctgccagc tggacaccat ctgcaagaac atcctgacca
     2281 gcagcaccga catcaccatc ttcagcccca acccccaggc catgcagaac ttcaacaagc
     2341 tgagcaccaa cgagaaagcc atctacctgg aatacctgtt cctgcccatg aagctgtacc
     2401 aggccgagat gaccctgagc cggcggatcc ccgtggacaa gctcgaggac gccatccggt
     2461 tcaagatcat cgaccagagc accttcatcg aggccagact gcccaaccag ggctacgtgg
     2521 ccgccagaat catcaacgcc aaccaggtgg ccaccaatgg catcgtgcac atcgtggatg
     2581 ccatccccgg cctgccctac gagacagtgc agcagtacgt ggcccggatc agcgacttca
     2641 ccctgtacta caataccggc ttcatccaga gcatgaccgt gggcgagccc tacaccttcc
     2701 tggtgcccac caatcaggcc atgaccagca tgaacaacga catcgaagtg ggcaacaagc
     2761 tgctgaccga caagctgcgg aaggacttcg tgttcagacg gaacaccttc cccctggacc
     2821 tgatcatcca ggacctggac atccggaagg tgtactacgt gtccaccgcc aactacgcct
     2881 tcaccagaga gcagctgcgg ctggaaatca actccaagaa cagcgacaag ggcgtgttca
     2941 cctaccggga cgagcggatc caggtgctga gcctgaacgg cccctaccag ttcaccaacg
     3001 gctttctgta cagcatcgac aagatcctgt acaccaagag cgacctgaac gccaccatgt
     3061 gcaccgtgtc cgcctgcgct ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     3121 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     3181 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     3241 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     3301 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     3361 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     3421 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     3481 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     3541 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     3601 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     3661 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     3721 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     3781 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3841 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     3901 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     3961 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     4021 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     4081 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     4141 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     4201 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     4261 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     4321 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     4381 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     4441 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     4501 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     4561 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     4621 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     4681 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     4741 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     4801 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     4861 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     4921 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     4981 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     5041 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     5101 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     5161 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     5221 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     5281 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     5341 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     5401 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     5461 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     5521 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5581 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5641 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5701 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     5761 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     5821 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     5881 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     5941 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6001 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     6061 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     6121 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     6181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     6541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     6841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8161 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8221 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8281 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     8341 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     8401 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     8461 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     8521 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
