LOCUS       sequence-229180-        9393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_170550-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4557..4574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4626..4681
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4680..4699)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6917..6935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7003..7107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7108..7176,7177..7968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7326..7345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8139..8727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8628..8647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8881..8898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9015..9036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9051..9056,9057..9074,9075..9081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9089..9105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9094..9116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9113..9129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9113..9129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 atctgtgccc cagacagtgc ctgcctggca gctgctgcga ggtggaagag aacgacttcc
     1261 ggtgctgccc ctacgccaac ggcgtgtgct gcagcgacgg caagaactgc tgtccacagg
     1321 gcaccaagtg cgacatggaa ctgggccagt gccggtcctc caacggcgcc gtgctgctga
     1381 gcaagcacat gagcgagaaa gtgatcggcg acctgaaaac cagcgccctg gacgtggtgc
     1441 tgcggcggct gagcgccaac tacatcatgt gccccgaccg gctgagcgtg tgccaggaca
     1501 acaccacctg ttgccccatg agcggcgaga tctggggctg ctgccagcac gccaacgcca
     1561 tctgctgccc cgatggcgag cactgctgtc cccacggcag cgtgtgcgat atggtcaacc
     1621 acagctgcaa gcccgccaag agcaacgaga tccccgagat gaaggaaagc ttccccaccc
     1681 tgcacctggt gtccgcctct gcccctgcca gactgaaccc caacgtgaag cccagcaagg
     1741 ccagccggcg gaagaacatc caccagccca gcgccaagta cctgctggcc tacagcggcg
     1801 tcgtgtgccc cgatcccctg tacgagtgcc ccgacaacac aacctgctgc agctccagcg
     1861 acgagaagtg ggcctgctgc cccatccccc agaccgtgtc cttcctgggc gtgtccgaca
     1921 aggccctgca caagagcctg gaacagctgc acagcaacaa gaacagcagc tacagcgtgg
     1981 ccaacttcaa cctgacctgg gtccgactga gcatcctgaa cggcacccag ctgtgccctg
     2041 acgccaagag ccggtgcagc gacgagcaga cctgttgcaa gctgaaaacc ggcgagtggg
     2101 gctgttgccc cgtgaagaac gccgtgtgct gttccgacgg ggagcactgt tgccctaacg
     2161 gctacacctg tgacctgagc gtgggcgagt gcatcaagaa cagccacagc agcaacgaga
     2221 gcaagaagtt cagcagcaac accatcctga gcagcctgcc catcaagatc ggcagcaacg
     2281 ccctgtgtcc cgacagcgac gtgtactgct ccgacaacag cacatgctgc gagcacgata
     2341 agaaatgggg atgctgcctg ttccccaatg ccgtgtgttg ctccgatggc atccactgct
     2401 gcccccagaa caccatctgc gacgtgcagg aagagatgtg catcagcaag agcggcgaca
     2461 tgatcaccaa gctgcagctg aagtccaccc ccaaactgca cagcttcaag agcaacagcc
     2521 ggatcaacat ctgcagcgac atgaagtccg tgtgcatcaa cggcacctgt tgcagcaacg
     2581 tgaacgaccc cctgagcacc ctgtgctgcc cttacgagaa tgctgtgtgc tgctctgacg
     2641 gcaaacactg ttgtcccaag ggaaccacct gtgacatgat caacggcggc tgcatcatca
     2701 gccaggaagg cctgagcaag agcaactcca tccccctgct gaccaagatc cgggccctgc
     2761 acgtgaccag caagaacaac acccagagca tcgtgttcac ccagatctgc cctggcggca
     2821 aggccaagtg ccccgatagc tccacttgct gcaagctgag cagcgggaag tacgcctgct
     2881 gtcctatccc caacgccatt tgttgcgccg acgatatcca ttgctgccct cccggcacca
     2941 gctgcgacta caagaccctg agctgcgtga aggccaacaa cgaccacagc atcggcagcc
     3001 cctccgccaa tgagctgctg cggaacccca agctgctgtt caccggcgtg cgggaccacg
     3061 tgtgccctgg ccaccagagc acctgttccg atgaccagac ctgctgccgg ctgcccgaca
     3121 agagctgggg gtgctgccct ctgaagaatg gcgtgtgttg tggcgaccac ttccattgct
     3181 gtccagccgg ctccgtgtgc gacctggaca ccaagcagtg catcctgcag ctggaactga
     3241 accagtccac caccaagatc gtgtacgacg gcttcaccat gcacattgcc aacagaaaga
     3301 gcagcgacag actgtacagc cagcccgtga agtccatcag caccatcgac gccagcgtgc
     3361 aggccaagtg gtgcggcgcc tgtggcgata cctgggcctg ttgcccagac gccaccttca
     3421 acagctggtc ctgctgccca tacgtgggcg gcgagtgctg catcggccag aacatctgtt
     3481 gcccccctgg cagccggtgc ctgaacaacg gcaagtgcga gaagatctcc aagaacatca
     3541 tgaagtcctt cgcccccacc ctgatcccca gcatctctaa gaacaacaac ctgcagaagt
     3601 cctacttcat ccagcagaac aacgtgaaag agaacttcca catcgagatg agcaatatcg
     3661 ccaagcccgc ccagcggaac atcctgctgc tcgtgcggca ctaccggcgg ctgaagcggc
     3721 agctgcacac aatctgcccc gacagcctgc actactgcgg caagagcgag cagtgctgcc
     3781 tgtccaacaa gttcggctac acatgcaccc ccaacaagag catctgctgc ggcagcaaca
     3841 tggacaccta ctgccccatc tacaaggact gcagcctgga cggcaagttc tgcatcgata
     3901 agtatggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     3961 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     4021 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     4081 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     4141 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     4201 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     4261 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     4321 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     4381 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     4441 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     4501 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     4561 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     4621 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     4681 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     4741 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     4801 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     4861 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     4921 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     4981 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     5041 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     5101 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     5161 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     5221 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     5281 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     5341 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     5401 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     5461 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     5521 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     5581 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     5641 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     5701 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     5761 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     5821 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     5881 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     5941 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     6001 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     6061 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     6121 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     6181 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     6241 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     6301 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     6361 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6421 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6481 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     6541 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     6601 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     6661 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     6721 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     6781 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     6841 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     6901 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6961 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7021 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7081 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7141 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7201 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7261 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7321 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     7381 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7441 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7501 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7561 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7621 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     7681 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7741 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7801 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7861 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7921 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7981 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8041 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8101 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8161 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8221 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8281 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8341 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8401 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8461 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8521 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8581 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8641 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8701 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8761 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8821 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8881 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8941 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     9001 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     9061 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     9121 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     9181 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     9241 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     9301 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     9361 acatttatat tggctcatgt ccaatatgac cgc
//
