LOCUS       sequence-229181-        7341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_035040-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2505..2522
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2574..2629
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2628..2647)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4865..4883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4951..5055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5056..5124,5125..5916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5274..5293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6087..6675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6576..6595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6829..6846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6963..6984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6999..7004,7005..7022,7023..7029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7037..7053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7042..7064
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7061..7077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7061..7077
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 atttcgtgct gcggcccacc aaccagatca cccacgccgg cagcaccatc cagcacagct
     1261 gcagcgtgat gaccgtgccc gtgttcatcg agtggtatct gaacaccaac aagatcggca
     1321 gctgcctgtt ccagaccaac accaccacca acaccttcgg cgaccccaag ttccagatca
     1381 tcatcggcca gtacaccgag aagctgctgc ccatctgcca gctgatcgtg accaacatcg
     1441 acttcgtgga caccggcgag tacaagtgca agaccatcca ccacgacagc gagagcgaca
     1501 ccgccagcgc ctacatcctg gtgtcctacc ccatccggaa gctggaactg cacatcgaca
     1561 acgagacaag cctgagcgtg ggccagcgga cctacatcga gtgtcaggcc agagccggca
     1621 agcctgcccc ccagatcaga ctgaatctgg gcggcctgcc catcagcgag gcccgggtgg
     1681 tgcagaaggt ggacatcgag ggcctgatca ccagcaccgc caccgccaat atcaagctga
     1741 ccaataccca ccactggcag ctgctgacct gccacgtgga catgcaggcc taccggaacg
     1801 tgaaccagac cttcaaagtg atccacctga aggactacgc ccctgccgag ctgggcgcgc
     1861 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     1921 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     1981 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2041 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2101 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2161 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2221 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2281 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2341 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2401 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2461 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     2521 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2581 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2641 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     2701 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2761 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2821 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2881 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2941 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3001 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3061 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3121 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3181 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3241 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3301 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3361 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3421 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3481 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3541 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3601 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3661 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3721 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3781 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3841 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3901 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3961 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4021 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4081 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4141 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4201 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4261 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4321 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4381 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4441 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4501 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4561 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4621 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4681 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4741 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4801 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     4861 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4921 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4981 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5041 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5101 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5161 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5221 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5281 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5341 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5401 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5461 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5521 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5581 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5641 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5701 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5761 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5821 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5881 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5941 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6001 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6061 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6121 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6181 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6241 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6301 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6361 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6421 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6481 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6541 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6601 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6661 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6721 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6781 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6841 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6901 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6961 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7021 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7081 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7141 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7201 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7261 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7321 gctcatgtcc aatatgaccg c
//
