LOCUS       sequence-229182-        8088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_052660-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3252..3269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3321..3376
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3375..3394)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5612..5630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5698..5802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5803..5871,5872..6663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6021..6040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6834..7422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7323..7342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7576..7593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7710..7731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7746..7751,7752..7769,7770..7776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7784..7800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7789..7811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7808..7824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7808..7824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcaactgcct gcagcggaag tacgacaaga acaccgtggc cagcatcccc ggcgacatca
     1261 tgctgggcgg cctgttccct gtgcacacca gcggcgtgac cacatgcgag agcatcaacc
     1321 ccgagcgggg catccagcgg gtggaagcca tgctgttcac cctggacgag atcaacaaca
     1381 acagcaagct gctgcccggc ctgaccctgg gcaccaccat cagagatacc tgcagcgaca
     1441 ccaaccacgc cctggaacag gccctgaagt tcgtgcaggc cagcaccggc agcagcagcc
     1501 agctgaacaa gatgaacgag cacagcgaga acctgaacac cagaggcgtc gtgggcagca
     1561 gctacagcag cgtgaccatc ctggtggcca acctgttccg gctgttcagc ctgccccaga
     1621 tcagctacgc cagcaccacc gccatcctga gcgacaagcg ggccttccct ctgttcgccc
     1681 ggaccgtgcc ttccgacgtg gtgcaggccc aggccatggc cacactggtg tccgcccaca
     1741 actggaccta cgtgtccacc gtgcgctctg ccggcgacta cggcgacagc ggcatggacg
     1801 ccttttggaa agaggccgac aagctgggcg tgtgtatcgc cgccagagaa gtgatccggg
     1861 gcaacaccgg ccctgaggac ctggacaaca tcgtgaacgt gctgagcaag gacttcgcca
     1921 aggcccgggt ggtggtgctg tttaccggca tggaccacac aaagctgctg ctggacgccg
     1981 tgcggagagc cggcctggtc aaccacttcg tgtggatcgc cagcgacggc tggggcagag
     2041 aaaacgtgcc cgtgtccaac aactccagag aggccaacgg cgccctgacc atcgagatcc
     2101 aggccgagcc catcaaggcc ttcaccgact actacctgag cctgggcaga gagaaccacc
     2161 ggaacccctg gttcagagag tactggcagg acctgctgca gtgcagagag agcagcacca
     2221 agcggagaga gcggaagtcc accagcagcc ggcggtactt catgagcaag ctgagcaaga
     2281 acgagacaaa cctggaaaac agccacgaga tcagcaccga gagcagctcc ctgcccagca
     2341 gctgcagcga ctcccccaac ctgaagctgc gggacatcct gggccccgac ttcaagcagg
     2401 aagccaagat ccagttcgtg tacgacgccg tgtacgcctt cgccagcggc ctgcacatcc
     2461 tgcagaaaaa gctgtgcccc ggcaaccccc accaccccaa gtggaacaag cggagctgcc
     2521 tggaaaagat gctgagctac cccggcaccg ccttctacca gctgatcatc aacaccagct
     2581 tcaccggcga gttcatcaga ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     2641 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     2701 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     2761 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     2821 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     2881 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     2941 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     3001 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     3061 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     3121 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     3181 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     3241 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     3301 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3361 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     3421 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     3481 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     3541 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     3601 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     3661 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     3721 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     3781 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     3841 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     3901 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     3961 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     4021 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     4081 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     4141 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     4201 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     4261 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     4321 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     4381 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     4441 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     4501 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     4561 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     4621 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     4681 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     4741 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     4801 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     4861 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     4921 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     4981 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     5041 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5101 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5161 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5221 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     5281 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     5341 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     5401 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     5461 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5521 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     5581 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5641 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     6061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     6361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7621 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7681 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7741 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7801 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     7861 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     7921 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     7981 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     8041 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
