LOCUS       sequence-229183-        7407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_128590-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2571..2588
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2640..2695
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2694..2713)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4931..4949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5017..5121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5122..5190,5191..5982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5340..5359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6153..6741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6642..6661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7029..7050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7065..7070,7071..7088,7089..7095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7103..7119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7108..7130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7127..7143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7127..7143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 aagagaacca gggcgacaac gactggtcta tgatcatgga agtggaagtc gaggacccca
     1261 cctacaacag cagcagcgac aagaccgacg gccggaactt catcatcaac ggcaagaaga
     1321 accagccctt cctgcagaac aacaacgaga cagtgaccga gcacgccacc agccagtaca
     1381 gcatcagcat ccctggcgct accatctgcg gcagctttct gtgctacaac cagggccagt
     1441 gctacaccaa cctgcacagc agctccagca agtgcctgtg ccccatcggc tacagcggcg
     1501 acttctgcga caaggtggac gagggctacc agttccccta catcgagcag ggcggctacc
     1561 tgaagttcta cttcaagagc agcgccctgt acagcggcat caaccagagc ttcatcgagc
     1621 ggagcaacca ggtgtccatg aacagcacca acacctataa ggtggacctg gaaatccggc
     1681 ccagcctgac cgacggcagc tacctgctgg tgtcccacga gagcaacaag ggcgacttca
     1741 agttcagcct gagcatcgag aacggctacg tggtgtacag aagcctgcgg atcagcaaga
     1801 ccaactacga gccctaccgg aaagaggaaa tccaggaagt caagcacttc acctttctga
     1861 acaccctgaa gaacaacatc atcagccacc tgggcagacg gctgatggaa aaagtgtctg
     1921 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct
     1981 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg
     2041 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga
     2101 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac
     2161 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga
     2221 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt
     2281 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga
     2341 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag
     2401 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg
     2461 ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc cagaagatgc
     2521 tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc caccaccatc
     2581 accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     2641 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2701 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     2761 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     2821 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     2881 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     2941 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     3001 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     3061 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     3121 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     3181 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     3241 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     3301 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     3361 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     3421 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     3481 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     3541 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     3601 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     3661 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     3721 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     3781 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     3841 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     3901 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     3961 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     4021 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     4081 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     4141 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     4201 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     4261 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     4321 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4381 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4441 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4501 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4561 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     4621 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4681 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4741 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     4801 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     4861 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     4921 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4981 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5341 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5641 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     5701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6841 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6901 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6961 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7021 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7081 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7141 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     7201 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
     7261 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
     7321 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
     7381 atattggctc atgtccaata tgaccgc
//
