LOCUS       sequence-229184-       11379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_135210-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..11379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6543..6560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6612..6667
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6666..6685)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8903..8921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8989..9093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9094..9162,9163..9954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9312..9331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10125..10713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10614..10633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10867..10884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11001..11022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11037..11042,11043..11060,11061..11067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11075..11091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11080..11102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11099..11115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11099..11115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 ttcagcagca ccagcagcag cagaaaaacc agaaccagaa gatcgaccac aagctgaagc
     1261 ggaacaacct gatgaacaag accagccagc tgagccggtt caaccatcag cagcacagct
     1321 accacgtgca gcccaagatg atgaacgtga acctgaacga gagcgacgag atgaagaacg
     1381 ccaccaagga cgtgatcaac ctgatcaccg gcctgaagaa caacaacaat gacgacgacg
     1441 atgagacaag cctgatcgac gaagaggaag atatggaact catcgacgag ctgaaagagt
     1501 acatgcccaa cgtgtgcttc gagacaaacc ccaagccccg gcagtaccgg cagcggatcc
     1561 agatccccta caccaagctg accagagtgt gggagcccaa ctgcgagaca gagggcaact
     1621 ggtgcatcaa gtacaaagag cagacccact acgccgtgca ctacaagacc gtgttcaaga
     1681 ccgcccccac caagcagctg cggtgctgcc ctggcttcac cagacagcct ggctacgagg
     1741 gctgcgtgcc cgtggccagc gactgcaacg agctgaacaa ctactgccag aacgactgcg
     1801 gccagatccc cggcagccgg aactgcagat gcccccctgg ctacttcctg gcccccgaca
     1861 agcggcggtg cctggacatc gacgagtgca cctacgacag aggcggctgc atgtactttt
     1921 gcgagaacct gcctggcggc tacagatgcg cctgccccag cgatatggtg ctggcccctg
     1981 ataagatcag ctgcatccca ttcttcaacc cctgcgagca cccccccaac ggcgactgcg
     2041 agcacatctg taccagcctg cccggctacc ggtacatgtg cagctgcaag ccaggctacc
     2101 acctggccga gaacaagaag cggtgcctgc ccgacaaccc ctgccagatc aacaatggcg
     2161 gctgccagca cgagtgccac aacgtgaacg gcgtggtgca ctgcagctgc cacgagggct
     2221 accagctggt gcctcacgac agcaagaagt gcaccgatat caacgagtgc agcatgaaca
     2281 acggcgggtg tcagcagctg tgccggaacg tggacggcag ctacagctgc gactgcaatc
     2341 ccggctggca gatcggcaac gacggcaaga gctgctacca ggtgtccgac ccctgccgct
     2401 tcgacaacgg cggctgcacc cacatctgca gcagaaccga tagcggcacc gccgagtgca
     2461 gctgctcccc cggatacgat ctgcaggccg acggcagaac ctgcagcgac cgggatgagt
     2521 gcgtgatcgg cgaggccaag tgctacggcc aggccgtgat ctgcatcaac gagcccggca
     2581 catacagatg cgagtgccag accggcttcc ggatcagccc cgatggccac agctgccagg
     2641 atatcgatga gtgcctggac gacaatggcg gatgcgagtt ccagtgcgtg aacaccatcg
     2701 gcggccaccg gtgcctgtgc gacttcggcg acgaactgaa ccccgacggc cggacctgct
     2761 cccggtctag cagctacgtg cgggacgaca gcagcaccct gttcacccct accaagcaga
     2821 accggaagca gctgaacatg aagcccaccc agcggatcgt gatcggggac gtgcaccagt
     2881 tcagcaagcc ccggaccagc ttcgacagcc ggggctctct gtgcctgcct ggcagattcg
     2941 gacacgactg cagcttcacc tgtagccact gcgagaacgg cggcagatgc aaccgggacc
     3001 tgaccggctg cgagtgctcc agcggctaca agggcatcat ctgcgaggaa aagtgcccca
     3061 agggcctgtg gggcgtggac tgcagacagt actgcgactg cgccaacgac gtggactgcg
     3121 actccgtgac cggcgtgtgc cagtgccctc tgggcagaca cggcaccaga tgcgagctgt
     3181 ctggctgcca ggccggcttt tggggccctc agtgcgacag actgtgcccc aaccactgcg
     3241 ccaccaacca gtgcgataga gagaagggcc actgtacctg cagccccggc acctacggca
     3301 gccactgcca cctgagctgc cccgagaata cctggggcga gctgtgcagc ctgaagtgcc
     3361 ctgccgagtg tctggacagc agacggcaca ccaagggctg cgaccctcag accggcgagt
     3421 gtatctgcaa gcccggatac cagccccccg actgcatcct gccctgccct cccagctact
     3481 tcggcttcaa ctgcaagatg acctgcttcg actgccagaa tggctgtgac cctgtgaccg
     3541 gccggtgcct ggtggaatgc cctcctggca agcacggcct gacctgcgag caggactgcc
     3601 ctccaggcta ctggggcaag cagtgctccc ggcactgtag atgcggctac gacgagaagg
     3661 gcaacatccg gacctgcgac cccaccagcg gcacctgttc ttgcaaggcc ggctacagag
     3721 gcaagaactg cgacgagccc tgcgagatcg gcacatacgg ccccaactgc cggctgaagt
     3781 gccagtgcgg catcagcaac atcggctgcg atcccgtgac aggactgtgt cagtgcccca
     3841 acggcatcgt gggccacaag tgctccatgc cctgcccacc cggcagatgg ggcaagtcct
     3901 gcaccgagat ctgcgattgc gagaacagcg tggaatgcca caagaacacc ggcctgtgca
     3961 tctgcttccc cggcttcatc ggcaacaagt gcgaccagaa gtgcgagaag gggaagtacg
     4021 gcctgaactg caccgaaacc tgcgcctgcg tgcacggcac ctgtgaccac agatctggcg
     4081 cctgcgagtg cgagcctggc tggcggaaca tgctgtgcaa caagccctgc gcccctggct
     4141 tctacggcgc cgattgccag cacagatgcc tgtgcaccaa cggcgccgag tgcgacccag
     4201 tgaccgggga ctgcgagtgc aacgaggggt ggtacggccc tgcctgcgac cagccttgta
     4261 gcgacggcac ctatggcaag aattgcgtga agaagtgcaa ctgcgccgac gtgcccggcg
     4321 tgtcctgcga caaagtgacc ggaagatgca tctgtccccc tggccggacc ggcaatgagt
     4381 gcagcgagct gtgtcccagc ggcttctggg gcgagggctg ctccagaacc tgtgcctgtc
     4441 tgagaggcgc cacatgcgac cctatcaccg gcaagtgccg gtgcagcagc gggtggtatg
     4501 gcgaggcctg cgagctgcct tgtcagagcg gccactgggg cgagtcctgt agcgtgccct
     4561 gtcagtgcag cgccaacacc agagatcccg agggcggcga gtgcgatcgg ttcacaggcg
     4621 agtgcagatg tccccagggc ttcaccggcc ccagatgcca ggacaagtgc cccccaggca
     4681 gatacggcag cgattgcaag ctgagctgtc cctgccagaa acctggcagc agatgcgatc
     4741 ctgtgactgg cgcctgtgaa tgtccccccg gatggttcgg cgagacatgc gagaggcctt
     4801 gcctgctggg cttctatggc accaactgca agcagcggtg cagctgccct agcggccagc
     4861 cttgtcacca cgagacaggc ctgtgctcct gccccgctgg caaatacggc ccacagtgcg
     4921 aggatacctg ccccgaggga agatggggcg aggactgtca gcacatttgc gattgccacc
     4981 tcaaaaacaa caacatgctc tgcgatccca tccacggcgt gtgctactgc cggcctggat
     5041 ggctgggccc caattgcatc agccggtgcc cagccggcta ctttggaatc aactgtcagc
     5101 atgagtgcaa gtgcaagaac aatggcgtgt gtcaccccga caacggcaca tgtatctgcc
     5161 aggctggcta ctatggcgag cggtgccagc tgaactgtcc tcccggcttt tacggcctgc
     5221 agtgcaagca gaagtgcggc tgtatccacg gggaggcctg caacgagatc accggggagt
     5281 gctactgctc cgccggctac cacggcaaga agtgtgaaca gacctgcctg ccagggacct
     5341 acggccccca gtgtagcctg atctgccccg tgtgccacac ccggatcgcc gactccaaca
     5401 ccctggacag cctgctgctg agcgagacaa gctctatccc cgacaacacc ttcctgagca
     5461 gccagtacca cagcgaagtg atgaagaaca ccaaggtggc catcctgcgg cggctgaacc
     5521 agaatcagaa caaccctacc tgtcaccccc agaccggaca gtgcccctgt cctcctggca
     5581 gcgagggcaa ggattgcagc accccctgcc ccaaggaccg cttcggccct gattgcgccc
     5641 agttcgtgct ggtggtcatc ctggggaagt gcgtgcacaa gctggtcaac atcgtgaagt
     5701 ccgtgatcag ccggctggac agcgtgtacg cccactggga catcatggaa aagcacgccg
     5761 ccgtgtacgt gcacgccgtg aacatgggcc tggtggtgcc caacttcgcc agagtgaaaa
     5821 ccatgcagtt cgtgcagctg atcctgggca ccgtgatcgt gtgcctggac ctgctggatc
     5881 tgaccgtgca gggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     5941 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     6001 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     6061 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     6121 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     6181 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     6241 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     6301 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     6361 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     6421 ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac atcctggacg
     6481 cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc cctctgggcc
     6541 cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc ccccgacctc
     6601 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     6661 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     6721 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     6781 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     6841 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     6901 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     6961 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     7021 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     7081 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     7141 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     7201 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     7261 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     7321 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     7381 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     7441 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     7501 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     7561 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     7621 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     7681 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     7741 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     7801 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     7861 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     7921 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     7981 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     8041 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     8101 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     8161 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     8221 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     8281 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     8341 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8401 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8461 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     8521 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     8581 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     8641 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     8701 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     8761 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     8821 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     8881 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8941 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9061 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9121 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9181 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9241 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9301 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9361 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9421 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9481 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9541 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9601 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     9661 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9721 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9781 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10981 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    11041 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    11101 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
    11161 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
    11221 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
    11281 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
    11341 atatgtacat ttatattggc tcatgtccaa tatgaccgc
//
