LOCUS       sequence-229186-        8271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_144130-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3435..3452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3504..3559
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3558..3577)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5795..5813)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5881..5985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5986..6054,6055..6846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6204..6223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7017..7605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7506..7525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7759..7776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7893..7914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7929..7934,7935..7952,7953..7959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7967..7983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7972..7994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7991..8007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7991..8007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tggacatcca gaaggacatc tacctgaacg accggaacat cagcgtgctg caggtcaacc
     1261 ccttcgccaa ccccagcgac tacatcaccg agacaatgaa ggtcaaacgg ctgaacaaga
     1321 gcaagctgag caacagcctg aagtcccaga gcctgcacag acggggcgac ctgatcgaga
     1381 gcgaccagaa cgacatcatc aagatcaagg ccgtgctgaa caacaccgcc ctgctgacct
     1441 gcagacccga caagaagctg ctgaccgaga acggcatcga cgagaacacc aagggcaccc
     1501 tgctgtggaa gcggaccaag accaacgagt acctgatcca caacaaccgg cggctgcacc
     1561 ccgacacccg gtacatcctg gacatgagca gcttcgacca gaccatcctg gacctgcgga
     1621 tcgaccacgt gcagcggacc gacgagggcg agtacgtgtg cctgtacagc aacggccaga
     1681 ccgtgtacaa gcagcgggtg tacctgaacg tgctggtgcc ccccgtgatc agcgagaaca
     1741 gcagcagccc caccagagtg atcgcccacg agggggaggc cgtgatcctg cagtgtaaag
     1801 cctggggcgt gcccgagccc atcatcacct ggtatctgac ccccaaagag ggccggcagt
     1861 accggatcta cgaggccacc gacaagagat tcgtgatcaa gcccaaccag ctgatcatca
     1921 gcaacatcac ccgggacctg cagggcacct acaagtgcct ggcccagaac ggcctgaaag
     1981 agaacacctg gcgggtcatc gagctggacg tgcgctaccc ccccaccatc gagatggcca
     2041 acctgaagct gggccagctg ctgaagggca ccaccatggt ccgagccgtg atcgccggca
     2101 accccatcaa cctgttctac tgggagttcg agggcagacc catccacggc cccaacagca
     2161 actgcctggt gcccatgccc aacgagaagt actgcatcct ggtggacaag gccaacagca
     2221 agcacggcat catccggacc accctgttta tcgccaacct gaccatggcc aactacggcc
     2281 actacacctg tgtggtggaa acccccttcg gcaccttccg gaacagcacc gagatcttcc
     2341 ggacctacag cagcccctcc accacctggg acaacagctt cggcggcaac tacgaccacc
     2401 acttccccaa cctgctgacc aactaccagg gcagctacga gaagatgagc gagaagctga
     2461 acaagcgggc cacctacctg acccaggacc ggctgaagcc cagcagctac agctccctga
     2521 gcagcgtgac cggccagaac gacttcaaca gcatctccac caccaacacc gtgaaccagc
     2581 ggcagccccc catctgccct acctgcctga agtacaagaa cgtgaaggac ctgaccaccc
     2641 tgagccccga ccccatgacc ctgaacacct tctccgacta caactacctg cggaacctgt
     2701 ccaacaccct gaagctgagc ctgagcacct tccacctgat ctggctgcac atcttcacca
     2761 gctttctgca ctactgcttc gtgggcgcgc cgtcgacctc catcacggcc tataagagtg
     2821 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2881 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2941 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     3001 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     3061 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     3121 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     3181 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     3241 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     3301 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     3361 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     3421 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     3481 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3541 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3601 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3661 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3721 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3781 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3841 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3901 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3961 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     4021 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     4081 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     4141 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4201 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     4261 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     4321 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4381 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4441 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4501 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4561 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4621 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4681 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4741 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4801 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4861 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4921 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4981 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     5041 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     5101 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     5161 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     5221 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5281 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5341 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5401 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5461 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5521 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5581 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5641 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5701 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5761 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5821 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6001 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6061 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6121 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6181 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6241 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6301 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6361 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6421 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6481 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6541 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6601 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6661 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6721 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6781 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6841 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6901 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6961 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7021 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7081 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7141 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7201 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7261 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7321 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7381 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7441 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7501 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7561 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7621 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7681 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7741 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7801 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7861 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7921 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7981 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     8041 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     8101 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     8161 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     8221 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
