LOCUS       sequence-229189-        8196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_178740-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1899..1908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3360..3377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3429..3484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3483..3502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5720..5738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5806..5910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5911..5979,5980..6771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6129..6148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6942..7530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7431..7450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7684..7701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7854..7859,7860..7877,7878..7884)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7892..7908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7897..7919
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7916..7932
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7916..7932
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 agccttgttc tgatgcccag gccgtgatcc agcagcggag ccagaacctg aagcccctga
     1261 ccaccatccg gcccttcgtg cccacagtgc ccggcatggc catcaacacc aacaccgccg
     1321 tgcagtacgt gcggaagccc gccgagatct ctgtgctggg agccgccgga accttcgtgg
     1381 ccacccccat gaaccagacc ttcaccccct tcgtgaaccc catcatgaac ctgaacccca
     1441 gcctgatcag cagcatccct ggcggcccta caggcggccc tggacaggtg tcatctctga
     1501 gcgccacagg cggcgtgctg agcagcctgg ccaacagcag aaagccctgc aactgcacca
     1561 agagccactg cctgaagctg tactgcgagt gcttcgccca gggccagctg tgccagaact
     1621 gcaattgcaa caactgcatg aacaacctgg cctacgagga agagagaggc agagccatca
     1681 agatgaccct ggaacggaac cccaccgcct tccaccccaa gatcggacgg ggcgagggcg
     1741 agagaaagca caccaagggc tgtaactgca agcggagcgg ctgcctgaag aactattgcg
     1801 agtgctacga ggccaagatc agctgcagcg acctgtgccg gtgccagggc tgcagaaaca
     1861 ccgaggacag cgtggaacgg cggagcctga tgagactggc cgccatgggc gccctgagaa
     1921 gcagacagcc cagcagcttc aagaagcacc tggtcaagcc ccccagcgag acactgcccc
     1981 acgtgttctt cacctgggaa gtgatcgagg ccaccgccag ctgtctgctg gcccaggccg
     2041 atgaggccga gagaagagat ctgccccctg ccgcccagga acggatcctg ctggaagagt
     2101 tcggccggac cctggaaaga gtgattgagg ccgccagcaa ggcccagatc agaagcagca
     2161 gcggcatcca caccagcgcc agcatcgacg acaacgacca gggcctgatc cactccggcg
     2221 gcagcacagg ccctggatct ctgggcggaa agggctctgg cgccgtggga gtggctgctg
     2281 ccgctgcagc tgctgccgac gtgctgaggg acgacgagga tgacgaggac gagatgatgc
     2341 acgacatcga cgtggaagag gaagaggacg accccgacga gatggacgac gagatgtacg
     2401 gcatcgtggg cggacccacc atcagccggc acggccacag cagaagccac ctgggcagac
     2461 ggcacatccc cagcagccac agatccaccc acctggtgcc cgagctggaa gatgacatca
     2521 tgagcgacga ggaccccgtg gaaagggccc acgccgtgtc ctccagcaac agacaggcca
     2581 tcatgcccca cagataccag gaaatgcaca gccggcggac cagccagacc gtggaagatc
     2641 ggtacatcca cagcaggcgg agcatgagca gcagaagcta ccggcaccac gaccccgata
     2701 gcatggtcgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2761 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2821 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2881 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2941 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     3001 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     3061 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     3121 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     3181 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     3241 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     3301 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     3361 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     3421 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3481 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     3541 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     3601 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     3661 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     3721 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     3781 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     3841 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     3901 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     3961 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     4021 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     4081 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     4141 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     4201 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     4261 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     4321 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     4381 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     4441 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     4501 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     4561 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     4621 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     4681 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     4741 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     4801 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     4861 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     4921 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     4981 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     5041 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     5101 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     5161 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5221 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     5281 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     5341 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     5401 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     5461 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5521 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5581 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5641 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     5701 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5761 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5821 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5881 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5941 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6001 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6061 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6121 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6181 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6241 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6301 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6361 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6421 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     6481 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6541 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6601 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6661 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6721 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6781 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6841 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6901 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6961 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7021 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7081 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7141 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7201 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7261 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7321 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7381 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7441 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7501 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7561 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7621 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7681 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7741 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7801 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7861 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7921 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     7981 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     8041 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     8101 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     8161 tgtacattta tattggctca tgtccaatat gaccgc
//
