LOCUS       sequence-229190-        7500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_180600-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2664..2681
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2733..2788
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2787..2806)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5024..5042)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5110..5214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5215..5283,5284..6075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5433..5452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6246..6834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6735..6754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6988..7005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7122..7143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7158..7163,7164..7181,7182..7188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7201..7223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7220..7236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7220..7236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gccagtacga caacgacgat caccaccact actacgagcc cagcaagagc ctgcagagcc
     1261 ccaccatcct gaagcagatc gaggacgagt gcaacaagtg cgaccggaag aagtgcagcc
     1321 ccatcatcca gtgcaacgtg ggcatcgtgc gggacaagtg cggctgctgc gacatctgcg
     1381 ccctggaaga ggcccagctg tgcaacatcc ccaacgactt catcaacggc attctgaagc
     1441 agggcatcac ttggtacggc atctgcggca ccgacctgga atgccggaag cggaccgaca
     1501 tcgacagcaa gatcatcgag agccagagca tctgctactg cgtgaagtcc ggcaaagtgt
     1561 gcggcagcga cggcatcacc tacagcaagt gcaagatgaa cgccaccatc atcagcagca
     1621 acggcaccgt gatccccatc agcgagggcc cctgtcagac cgtgcccagc gtgaagctga
     1681 ccgtgctgaa ccagaccgtg atcgagggca ccaacgtgac cctgatctgc gaggccagag
     1741 gctaccccat ccccaccatc aactggtact tcaacaagcc caaccagaag caggacaaga
     1801 tccagatgcc cggcaactac aaggacgtga ccgtgtccgt gcggggaggc accgaggaaa
     1861 cccacaccat cagctacctg cagatcacca gcttcaccct ggaatacgag ggcgagtaca
     1921 tctgcatggc cgacaacatc gtgggcatca aggcccaggg caccagcctc gtgcggaacg
     1981 ccgccagcag catctacagc agcagcagct ctggcgcgcc gtcgacctcc atcacggcct
     2041 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2101 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2161 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2221 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2281 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2341 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     2401 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     2461 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     2521 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     2581 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     2641 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     2701 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     2761 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2821 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     2881 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     2941 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3001 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3061 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3121 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     3181 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3241 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3301 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     3361 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     3421 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     3481 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     3541 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     3601 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     3661 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     3721 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     3781 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     3841 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     3901 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     3961 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4021 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4081 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4141 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     4201 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4261 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4321 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     4381 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     4441 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4501 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4561 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4621 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     4681 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     4741 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     4801 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4861 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4921 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     4981 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5041 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5101 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5161 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5221 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5281 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5341 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5401 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5461 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5521 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5581 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5641 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5701 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     5761 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5821 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5881 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5941 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6001 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6061 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6901 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6961 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7081 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7141 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7201 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7261 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7321 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     7381 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     7441 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
