LOCUS       sequence-229191-        7155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_181220-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2319..2336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2388..2443
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2442..2461)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4679..4697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4765..4869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4870..4938,4939..5730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5088..5107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5901..6489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6390..6409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6643..6660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6777..6798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6813..6818,6819..6836,6837..6843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6851..6867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6856..6878
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6875..6891
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6875..6891
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 acaagggcac ccaggtgctg agcaagccca gcaccaccaa gcacctgacc acctccacct
     1261 ggttcagcag cagccaggtg gaccggcagc tgaaccagtt cctgaccaac gagatcaagc
     1321 aggaaaaggc caacgccttc ttctgcaacc cccccgaggg cttcaaggtg gtcaagaccg
     1381 acggctgcga catcgtgatc cggaaagagt accaggacgg cgtcagcgtg gacgtggaaa
     1441 tcaacctggc cggcagcgtg tcccccagca tccccgagga tgatgtgtcc gcccctgaga
     1501 agatggaaga tgacgccccc ctggaagccc accccgacct gcggatcaag ctgaccaagc
     1561 cctccggccg gtccctggtg ttcaactgca gcctgcccgg ccacgacgcc gacaagcagc
     1621 tgagcgagga cgagagcaac aacctgcgga agtggttcct gtgcattggc gcgccgtcga
     1681 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     1741 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     1801 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     1861 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     1921 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     1981 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     2041 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     2101 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     2161 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     2221 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     2281 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     2341 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2401 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2461 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     2521 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     2581 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     2641 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     2701 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     2761 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     2821 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     2881 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     2941 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3001 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3061 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     3121 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3181 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3241 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3301 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3361 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3421 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     3481 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     3541 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     3601 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     3661 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     3721 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     3781 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     3841 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     3901 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     3961 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4021 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4081 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4141 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4201 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4261 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4321 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4381 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4441 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4501 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     4561 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     4621 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     4681 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5101 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5401 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6541 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6601 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6661 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6721 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6781 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6841 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6901 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     6961 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     7021 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     7081 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     7141 gtccaatatg accgc
//
