LOCUS       sequence-229192-        8703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_211020-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3867..3884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3936..3991
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3990..4009)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6227..6245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6313..6417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6418..6486,6487..7278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6636..6655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7449..8037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7938..7957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8191..8208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8325..8346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8361..8366,8367..8384,8385..8391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8399..8415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8404..8426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8423..8439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8423..8439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgaacgagca cgtgcaggaa agcaccacca tcctgaacaa agagtgcgag aacgacgacc
     1261 ccctgggcat gatcagcggc tctatcgccg actggcagat caccagcagc agcacctacc
     1321 ccagcagctg ggtcaagggc tgcgaggaaa agaacgcccg gctgttccgg accaacggcc
     1381 tggcttggtg cgccaagttc aagagcagca gcgagtggct gcagatcgac ctgggcgtgc
     1441 aggccctggt caccggcatc atgacacagg gcagaggcga cggctccgag tgggtcacca
     1501 gcttcatggt gtcctacagc gacgacgcca tgcactggaa gttcgtgacc gaccagtacg
     1561 ccaaccagaa gatcttcgag ggcaacagcg acagctacag cgtgaagcac aactacttcg
     1621 acgagcccat ccgggccaga ttcgtgaaga tccacaccta cacctggcac aaccacccca
     1681 gcctgcgggt ggaactcgtg ggctgccaga gctgcaagca gatcatcggc atccccccat
     1741 acgccagatt cgccgccagc agctcccggg gcaagcggag ccagagaagc tgcacccccg
     1801 agtacggcca ctacctgagc aacaaggctt ggtgtgccca gcggcaggac gccctgcagt
     1861 ggctccagat tgatgtgggc cctcccaccc tgatcaccgg cgtgatcctg aagtccaggg
     1921 gcgacgtgaa gaacagccag tacgtgaccc ggttcaagct gagctacagc aacgacaccc
     1981 ggctgtggta cttctacaag gatgccgccc ctctggaccc ccggtccacc gtgtggtcct
     2041 tcgagtggtc ccagaagatg aaggaaagcg acgacaagat caagagcgac aagatttttg
     2101 aggggaacaa cgagcagatc accgagcgga tccactacct ggccaccccc tttatcgcca
     2161 gatacgtgcg gatccacccc gtgatctggc ggagccggat cgccatgaga gtgggcctgc
     2221 tgggatgcaa gcagaagggc agctgcagcg ccggcttctt ccggatcaac aacgagagca
     2281 gctgcgtgcc caacctggcc tacaagaaga atgcctggct gacccccgag agcagcaacc
     2341 accggaagcg gaacctgcag gacagcgaca aggccagcag aagccacggc ttcaccagcg
     2401 agccccggaa cgtgatcgac cccgagaacc ccagccactt ccagtacacc gagaaccaga
     2461 ggccccccat gctggcccac caccggatcc gggacaacac cgtgaacagc aacgccttca
     2521 agttcagcaa catccccctg tggtcccccg tgaacggcat gatcaacgag cagttcgtgg
     2581 gcgagagcag cgacggcatg gccctgctgg ccgtggatgg ctggaccggc gaggaaatcc
     2641 tgctgaagaa ctccaccctg agcacccggt ccagcgagcg gctgatgaac gacgcccccg
     2701 acaacaagca gctgaccaac gacagcctga acaagaaaac catccagaac gtggtgcaca
     2761 ccaccttcgt gacccagaag tccgcccaca gctgcaccat cctggaatac cggtggccct
     2821 tcgtggaact gccctcttgg tacgtggacc tgcgcgagca catcgaagtg aagggcgtgg
     2881 tcatctacac agccggccac ggccgggacg gcagatacct gctgagcagc ctgttcggct
     2941 ctgaggaaaa gaccaagttc aacagcgaga acctggaacg gctgagcatc tacgtggaaa
     3001 gcgagcccag acaccacgac accaagaccc tgagccggtc cagctccagc ctgtgcggct
     3061 tcgtgaccag gatcaacgac gccatcttca gccccagact gcacatcccc tgccggcagc
     3121 ccctgatcgg ccgctacgtg tacgtggaag ccagaggcgt gcggggcaga tggtcccagg
     3181 aattcgccgc cctgctgtgc gaagtgatgg tgtatggcgc gccgtcgacc tccatcacgg
     3241 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     3301 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     3361 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     3421 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     3481 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     3541 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     3601 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     3661 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     3721 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     3781 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     3841 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     3901 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     3961 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     4021 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     4081 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     4141 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     4201 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     4261 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     4321 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     4381 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     4441 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     4501 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     4561 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     4621 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     4681 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     4741 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     4801 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     4861 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     4921 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     4981 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     5041 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     5101 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     5161 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     5221 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     5281 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     5341 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     5401 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     5461 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     5521 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     5581 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     5641 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5701 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     5761 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5821 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     5881 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     5941 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     6001 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     6061 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     6121 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     6181 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     6241 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6301 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6421 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6481 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6541 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6601 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6661 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6721 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6781 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6841 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6901 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6961 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7021 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7081 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7141 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7201 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7261 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7441 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7501 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7561 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7621 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7681 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7741 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7801 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7861 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7921 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7981 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8041 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8101 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8161 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8221 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8281 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8341 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8401 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     8461 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     8521 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     8581 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     8641 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     8701 cgc
//
