LOCUS       sequence-229194-        7014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_130100-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2178..2195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2247..2302
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2301..2320)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4538..4556)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4624..4728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4729..4797,4798..5589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4947..4966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5760..6348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6249..6268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6502..6519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6636..6657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6672..6677,6678..6695,6696..6702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6715..6737
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6734..6750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6734..6750
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ttaaggtgtc caccgtggaa gagaacgact acgagatgca gaacgtgacc ctggaatgcc
     1261 ggctgtgcct gagcggcctg agcctggccc actacttcat ggacgacctg tactggcggg
     1321 acgtgtacat gatgggcgcc aagaagctgt gcaccttcat cagcagcgag cagatcaaga
     1381 acatctgcaa caagtacacc agcaagtacc tgcccaagat cctggacgaa gtgggcagcc
     1441 tgatcggccc tggcatctgc accgacttcg acgtgtgcaa gagcaccgaa gtgaagatgt
     1501 tcacaattca ggaaaacaac aagattggcg cgccgtcgac ctccatcacg gcctataaga
     1561 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     1621 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     1681 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     1741 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     1801 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     1861 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     1921 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     1981 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     2041 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     2101 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     2161 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     2221 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2281 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     2341 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     2401 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     2461 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     2521 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     2581 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     2641 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     2701 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     2761 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     2821 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     2881 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     2941 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     3001 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     3061 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3121 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3181 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3241 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3301 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     3361 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     3421 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     3481 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     3541 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     3601 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     3661 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     3721 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     3781 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     3841 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     3901 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     3961 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4021 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     4081 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4141 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4201 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4261 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4321 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     4381 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4441 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     4501 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4561 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5881 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6541 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6601 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6661 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6721 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     6781 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     6841 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     6901 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     6961 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
