LOCUS       sequence-229197-        7038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_202610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2202..2219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2271..2326
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2325..2344)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4562..4580)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4648..4752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4753..4821,4822..5613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4971..4990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5784..6372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6273..6292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6526..6543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6660..6681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6696..6701,6702..6719,6720..6726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6734..6750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6739..6761
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6758..6774
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6758..6774
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 tgaagtccga ggaatacgcc cagatgagca tcctgaacac cgaccagccc gactgccact
     1261 tcctgtgcga gctgtgcacc agcaccgtga acgccaccaa gtacatcctg gacaacgagc
     1321 ccttcatccc cgagatcctg aagaacttca tgcccatctg ctacatgctg cccaagctgc
     1381 agtaccggaa gatctgctac cacttcgtga acggcggagc cgtggacctg atccacgagc
     1441 tggtcaacac catcaacgag aacaatttct gcttccacct gggcctgtgc agctccacca
     1501 tcccctgccc cagcttccag aaaaccctga cctacaacga cctgagcaca ggcgcgccgt
     1561 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     1621 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     1681 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     1741 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     1801 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     1861 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     1921 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     1981 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     2041 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     2101 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     2161 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     2221 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     2281 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     2341 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     2401 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     2461 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     2521 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     2581 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     2641 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     2701 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     2761 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     2821 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     2881 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     2941 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3001 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3061 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3121 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3181 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3241 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     3301 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     3361 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     3421 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     3481 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     3541 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     3601 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     3661 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     3721 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     3781 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     3841 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     3901 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     3961 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4021 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4081 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4141 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4201 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     4261 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4321 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4381 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     4441 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     4501 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     4561 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4621 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4681 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4741 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4801 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4981 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5221 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5281 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5341 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5401 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5461 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5521 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5581 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5641 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5701 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5761 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5821 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5881 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5941 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6001 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6061 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6121 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6181 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6241 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6301 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6361 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6421 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6481 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6541 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6601 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6661 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6721 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6781 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     6841 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     6901 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     6961 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     7021 catgtccaat atgaccgc
//
