LOCUS       sequence-229198-        8364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_090100-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3528..3545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3597..3652
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3651..3670)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5888..5906)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5974..6078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6079..6147,6148..6939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6297..6316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7110..7698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7599..7618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7852..7869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7986..8007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8022..8027,8028..8045,8046..8052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8060..8076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8065..8087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8084..8100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8084..8100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agtacatctg ccagatcatg cccaacatca gcatgaactt cggcatcctg ggcgagccca
     1261 tcgacaagcg gcagtacggc ggcaaccggc tgaagttcat cgtgatctac agagagagca
     1321 tccagcggca cagcctgttc ggccggttca agagcgagat cgtggaaaag gccctgaact
     1381 tctgggagcg gaccctgagc gtgcggaggc cccccaacag aaagctgctg atcgaccggg
     1441 gctgcgtgga acccgtgttc tacaccgacg gccggaccgg caagaagttc tgcaagagcc
     1501 agtgcaagca gaaagccctg tgctacgacc accccgtgcc cgaccagtac gcctctggat
     1561 gtgctatggg cggaggcggc ggagacatcc gggacgtgta ccaggatggc cctggcttcg
     1621 cccccaacga gtacgtgctg ttcgtggaaa gcgagaacaa gcagggctgc ctgagcggcg
     1681 ccacactggc ctatgccggc ccttgcgaga tgcaccccac caccgaccgg cccatcatgg
     1741 gcagcatcaa cttttgtcca caaaagatgg aaatccagga acccggcaag accatgctga
     1801 tcggcaccgc catccacgag ctgggccacg ccctgggctt cgtgaagtcc aacttcgccc
     1861 tgatgcggga ccccgagggc aaccccagaa cccccagaga tcccaagagc ggcagacccc
     1921 ccctgaacaa cgagagacag tacagcgcca gcgagaacac cgtgcggatc atcaaccggc
     1981 cctgggtcac cgccgctggc accttcagca agcccttcat gagcttcgtg acccctgccc
     2041 tgctggaaga ggcccggaag cacttcaact gccccaacct ggacggcctg gacatcgaga
     2101 atgagggcgg ccagggcacc atcggcaccc acttcgagaa gcgggtcgtg ggcgacgaga
     2161 caatggccgg cgtgacaggc gtgaagtccg tgctgagccg gctgaccctg gccttcttca
     2221 ccgacagcgg gtggtgggac gtggactaca gcctggccga gccctggtac tacggcaaga
     2281 acctgggctg cagcttcgtg atggaaagct gctacgccta catgatgcgg atgaagcagg
     2341 ccggcaagag caccgagccc tactgcgacg agcccgacac cctgaagtgc taccaccaga
     2401 aggccttcgg catctgcgcc gtgggccggt tcacccagag actgcccccc accgagcagt
     2461 acttcaacga ccctacccag ggcggcacca gcgtgctgaa cgacagatgc cccctgatcc
     2521 agcctatgcg gagcttcttc aacgagcccc tggtcaccta ctgcgatcac cagctgaaca
     2581 tccccgtggc ccagcggggc aacatgttcg cccaggactt cggcaacaac agcatgtgca
     2641 tcatccacaa gggcgcctgg cgggctgaga tgaacggcag agccaccaac gaccccagag
     2701 tgaaggccac ctgtcaccag tacagctgct ctggcggcct gcaggtcatc atcaacggca
     2761 agcctttcca gtgcaacagc ggcgtggcca agatccagac caagcagatc cagggcgaga
     2821 tcatctgccc caatcccaat atcgcctgcc ggaacagacg gcggagcatc aagcaaggcg
     2881 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2941 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     3001 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     3061 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     3121 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     3181 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     3241 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     3301 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     3361 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     3421 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     3481 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     3541 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3601 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3661 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     3721 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     3781 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3841 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3901 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3961 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     4021 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     4081 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     4141 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     4201 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     4261 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     4321 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     4381 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     4441 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4501 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4561 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4621 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     4681 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     4741 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     4801 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4861 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4921 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4981 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     5041 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     5101 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     5161 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     5221 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     5281 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     5341 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5401 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5461 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     5521 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5581 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     5641 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5701 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5761 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5821 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5881 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5941 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6001 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6061 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6121 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6181 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6241 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6301 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6361 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6421 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6481 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6541 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6601 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6661 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6721 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6781 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6841 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6901 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6961 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7021 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7081 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7861 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7921 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7981 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8041 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8101 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     8161 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     8221 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     8281 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     8341 ttggctcatg tccaatatga ccgc
//
