LOCUS       sequence-229201-        7992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_019030-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3156..3173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3225..3280
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3279..3298)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5516..5534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5602..5706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5707..5775,5776..6567)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5925..5944)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6738..7326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7227..7246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7480..7497
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7614..7635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7650..7655,7656..7673,7674..7680)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7688..7704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7693..7715
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7712..7728
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7712..7728
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 atacccccgc caactgtacc tatgaggacg cccacggccg gtggaagttc cacatcggcg
     1261 actaccagag caagtgcccc gagaagctga acagcaagca gagcgtggtc atcagcctgc
     1321 tgtaccccga tatcgccatc gacgagttcg gcaaccgggg ccactggacc ctgatctaca
     1381 accagggctt cgaagtgacc atcaaccacc ggaagtggct ggtcatcttc gcctacaaga
     1441 gcaacggcga gttcaactgc cacaagagca tgcccatgtg gacccacgac accctgatcc
     1501 ggcagtggaa gtgcttcgtg gccgagaaga tcggcgtgca cgacaagttt cacatcaaca
     1561 agctgttcgg cagcaagagc ttcggccgga ccctgtacca catcaacccc agcttcgtgg
     1621 acaagatcaa cgcccaccag aagtcttgga gagccgagat ctaccccgag ctgagcaagt
     1681 acaccatcga tgagctgcgg aacagagccg gcggagtgaa gtccatggtc acccggccca
     1741 gcgtgctgaa cagaaagacc cccagcaaag agctgatcag cctgaccggc aacctgcccc
     1801 tggaattcga ctggaccagc ccccctgacg gcagcagatc ccccgtgacc cccatccgga
     1861 atcagggcat ctgcggcagc tgctacgcct tcgcctctgc cgccgctctg gaagcccgga
     1921 tccggctggt gtccaacttc agcgagcagc ccatcctgag cccccaggcc gtggtggact
     1981 gcagccctta cagcgagggc tgcaacggcg gcttcccctt cctgatcgcc gggaagtacg
     2041 gcgaggactt cggctttgtg tccgagaact gcgaccccta caccggcgag gacaccggca
     2101 agtgcaccgt gtccaagaat tgcacccggt actacaccac cgactacagc tacatcggcg
     2161 gctactacgg cgccaccaat gagaagctga tgcagctgga actgatcagc aacggcccct
     2221 tcccagtggg cttcgaggtg tacgaggact tccagttcta caaagagggc atctaccacc
     2281 acaccaccgt gcagaacgac cactacaact tcaacccctt cgagctgacc aaccacgccg
     2341 tgctgctcgt gggctacggc gtggacaagc tgagcggcga gccctactgg aaagtgaaga
     2401 acagctgggg cgtggaatgg ggcgagcagg gctacttccg gatcctgaga ggcaccgacg
     2461 agtgcggcgt ggaaagcctg ggcgtcagat tcgaccccgt gctgggcgcg ccgtcgacct
     2521 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     2581 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     2641 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     2701 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     2761 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     2821 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     2881 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     2941 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     3001 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     3061 tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg aaccacaggg
     3121 acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac cattgagtct
     3181 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     3241 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3301 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     3361 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     3421 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     3481 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     3541 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     3601 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     3661 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     3721 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     3781 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     3841 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     3901 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     3961 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     4021 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     4081 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     4141 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     4201 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     4261 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     4321 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     4381 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     4441 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     4501 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     4561 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     4621 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     4681 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     4741 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     4801 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     4861 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     4921 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     4981 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     5041 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5101 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     5161 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     5221 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     5281 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5341 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5401 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     5461 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     5521 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5581 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5641 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5701 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5761 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5821 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5881 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5941 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6001 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6061 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6121 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6181 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6241 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6301 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6361 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6421 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6481 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6541 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6601 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6661 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6721 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6781 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6841 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6901 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6961 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7021 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7081 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7141 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7201 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7261 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7321 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7381 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7441 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7501 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7561 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7621 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7681 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7741 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     7801 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     7861 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     7921 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     7981 caatatgacc gc
//
