LOCUS       sequence-229202-        8133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_002600-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3297..3314
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3366..3421
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3420..3439)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5657..5675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5743..5847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5848..5916,5917..6708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6066..6085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6879..7467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7368..7387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7621..7638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7755..7776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7791..7796,7797..7814,7815..7821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7829..7845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7834..7856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7853..7869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7853..7869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgctgaccag acacttcccc tggtccacca tcaagaaccc cgacttcacc tacgagacaa
     1261 agtacttctg gacccgggtg gaccacttca gcttcgtgaa cgacgagaag ttcctgatcc
     1321 gctacctgat caacaacgag agcttcaccc ctggcggccc tatcctgttc tacaccggca
     1381 acgagggccc catcgagaca ttcgccgaga acagcggctt catctggaag ctgagccgcg
     1441 agctgaacgc cagcgtggtg ttcgccgagc accggtacta cggcaccagc ctgcccttcg
     1501 gcaacaacag cttcaaggac cggcggcact tcggctacct gaccgccgag cagaccctgg
     1561 ccgactacgt gctgctgatc aaccagctga aggccaacta cagctgcttc gccagcagcc
     1621 ccgtgatcgc ctttggcggc agctacggcg gcatgctgag cgcctggatc cggcagaagt
     1681 accccaacca gatcgctggc gccattgcca gcagcgcccc tgtgtggctg ttccccggcc
     1741 tgagcgactg caacggcttc agcatgacca tcaccaacag ctttctgaag tacggcggcg
     1801 agaactgcgt gaagaacatc cagctgagct ggtccaacat cgtggacatc ggccagagcg
     1861 tggacggcaa agaactgctg acccacatgt tcaacatctg cacccccctg accgacgtgc
     1921 agaacatcat cgactacctg tccgacttcc tgggcatcat cagcatggtc aactacccct
     1981 accccgccag cctgatcctg gccctgcctg agtggcccgt gaagtacctg tgcaccaacc
     2041 tgagcgagta cgacccccag cagcccgtgg tcaccagaat cagcctgctg gccaaggccg
     2101 tgctgagcct gaccaactac acaggcaacc agacctgcct ggacatcagc atgaacctgc
     2161 ccaacgtgga cgtgaacggc tgggacctgc aggcctgtat ggaaatggtc acccctacct
     2221 gcgccagcgg ccctgtgaac atcatgcccc ccagcaactg ggacctgaaa acctacagca
     2281 tctactgcca gaacagattc ggcgtgtccc ccagagtgga atggcccaag gtggaattct
     2341 ggtccaagag cgtgtacacc gtgaccaaca tcatcttcag caacggcgag atcgaccctt
     2401 ggagcgcctt cagcatcaca aacaacagct acgtgccctt cgccaccgtg atcaatatga
     2461 gcgacgccgc ccaccacctg gacctgagaa cccccaacag cgccgacccc cagtccgtgg
     2521 tggaagccag agagatcgag aagcagaaaa tcatccagtg gatcaaagag tggaacctga
     2581 agtacaagct gctcaagatc ttcaacggca tcgtgtcctt catctccctg ctggactacc
     2641 tgggaggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2701 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2761 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     2821 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     2881 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     2941 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     3001 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     3061 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     3121 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     3181 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     3241 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     3301 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     3361 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3421 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     3481 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     3541 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3601 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3661 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3721 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3781 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     3841 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3901 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     3961 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     4021 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     4081 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     4141 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     4201 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     4261 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     4321 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     4381 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     4441 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     4501 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     4561 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4621 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4681 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4741 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4801 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     4861 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4921 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     4981 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     5041 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     5101 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5161 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5221 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     5281 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     5341 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     5401 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5461 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     5521 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5581 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     5641 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5701 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6061 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6361 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     6421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7621 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7681 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7741 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7801 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7861 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     7921 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     7981 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     8041 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     8101 acatttatat tggctcatgt ccaatatgac cgc
//
