LOCUS       sequence-229203-        8034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_071610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3198..3215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3267..3322
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3321..3340)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5558..5576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5644..5748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5749..5817,5818..6609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5967..5986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6780..7368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7269..7288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7522..7539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7656..7677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7692..7697,7698..7715,7716..7722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7730..7746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7735..7757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7754..7770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7754..7770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcccttggcc ccccaaagag aactacttcg accagaccct ggaccacttc aattttcagg
     1261 cccggaacct gaccttcaag cagagatacc tgtacgagga caagtggttc aagcccaacg
     1321 gccccatctt cttctactgc ggcaacgagg gcggcatcga cggcttttgg aacaacaccg
     1381 gcctgatctt cgagctggcc cccagcttca acgccttcgt gctgttcgcc gagcaccggt
     1441 actacggcaa gagcctgccc ttcaacacca gcttccagca gccctacatc cagtacctga
     1501 gcatcgatca ggccctggcc gactacgcct acctgatcga gggcatcaag agcaccttca
     1561 acatgacccg gtccctggtg gtggccttcg gcggctctta tggcggcatg ctggccgcct
     1621 acatgcgggc caagtacccc cacatcatca agggcgccct ggccagcagc gcccctgtca
     1681 gatgggtggc cggcgagggc aacttccacg acttcttcga gagcgtgacc aaggactacc
     1741 gggacgccga ccccaagtgc agcgagaaga tcaagaacgc cttcaacctg gccgtgcagc
     1801 tgagccagaa gcccgacatc ggctacaagc agctgagcaa cgacctgcgg ctgtgcaagc
     1861 ccatccagaa cgacttcgag ttctactggg tgctgaagtg ggcccggaat gccttcgtga
     1921 tgatggccat gctggactac ccctacaagg ccagcttcat ggccagcctg cccgccaacc
     1981 ccgtgaacgt gtcctgcaag aacgccctgg ccgtgaccga cctgatcccc accctgagag
     2041 aagccgtggg cgtgttctac aacagcagcc agtccctgcc ctgcttcgac tacaagaccc
     2101 agttcatcga gtgcgccgac atcaccggct gcggcctggg ctctgatagc ctggcctggg
     2161 acttccagag ctgcaccgag atgaacctgc acgacgacag cgacagcacc accaacgaca
     2221 tgttcgtgtc cctgcccctg accaagcagc aggtcacaag ctactgccag cggagatggg
     2281 gcgtgacccc cgccttcaat cagctgagca ccttctacgg cgacaacatc tggaaaacca
     2341 gcagcaacat catcttcagc aacggcaacc tggacccctg gatgggcgga ggcatcctga
     2401 ccgaccagag cgagaaagtg atcagcctgg tgctggacgg cggagcccac cacctggacc
     2461 tgagaagccc cgaccccaac gaccccccta gcgctagaca ggtccgacag atcgaggtgc
     2521 agaccatcag aagctggctg gacctgggcg cgccgtcgac ctccatcacg gcctataaga
     2581 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     2641 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     2701 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     2761 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     2821 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     2881 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     2941 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3001 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3061 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3121 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     3181 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     3241 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     3301 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     3361 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     3421 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     3481 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     3541 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     3601 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     3661 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     3721 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     3781 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     3841 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     3901 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     3961 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4021 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4081 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4141 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4201 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     4261 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     4321 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     4381 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     4441 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     4501 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     4561 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     4621 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     4681 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     4741 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     4801 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     4861 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     4921 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4981 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5041 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5101 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5161 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     5221 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     5281 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5341 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5401 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5461 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     5521 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5581 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5701 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5761 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5821 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5881 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5941 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6001 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6061 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6121 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6181 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6241 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6301 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6361 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6421 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6481 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6541 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6601 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6661 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6721 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6781 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6841 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6901 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6961 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7021 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7141 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7201 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7381 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7441 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7501 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7561 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7621 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7681 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7741 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     7801 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     7861 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     7921 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     7981 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
