LOCUS       sequence-229204-       13014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_089670-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..13014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8178..8195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8247..8302
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8301..8320)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10538..10556)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10624..10728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10729..10797,10798..11589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10947..10966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11760..12348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12249..12268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12502..12519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12636..12657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12672..12677,12678..12695,12696..12702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12710..12726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12715..12737
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12734..12750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12734..12750
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gcaccgacgt gcacatcacc agcagcaacg agaacccctt caagaccgat gtgcagcagc
     1261 tgaacatcac caacgacagc aacaacaccg agacaatcag cagcaccctg taccccaacg
     1321 tgacctacgt gatcctgccc accggcttct acctgggcca gaccaacctg atcaccgtgc
     1381 ggagcagaga gccccggacc cagctgcgga tcagcatgaa cgtgaccgag ctgccccacg
     1441 agatcaacga ccccgaccag atctactacc tggaacccct gaacaccacc gagtattggt
     1501 acggcatcga tatcccttac gagatccctt tcatcttcac ccacgaggac tctatgtggg
     1561 tcaagctgat cttccacttc acccactgcc tgaacaagac cgacgtgatc cccaccgaca
     1621 acgagtgcac cacctacaag accaccgaag tgatccacag catccagctg aagcagcccc
     1681 ccatgatcat cctgggcgag acagacaagc ccatctacag acccggcgag aacgtgcggt
     1741 tccggttcct gaccctgaac gtgaacagcc tgctgcccag aagcaccagc atccccctgc
     1801 ccaagaagaa gctgatcatt cacaagggca gcaagggcca gctgatcgag atgaacaagc
     1861 tggaactgat gaagtggaac aacatcatct acgactccat cgtgatcatc gaccccatgg
     1921 acaaccacgt gaagcagtgg ttcaacatca gccccaacca ggccatcaac ctgacctacc
     1981 ccatcctgca caacgccaaa gagggccagt ggaagctgcg ggccaccgtg cggcaccact
     2041 acatcgagac aatcgagttc acagtgaagc agtacaccct gcctaagttc accgtgtccc
     2101 tggaaacccc cagagtgttc aacatggaaa gcgagtacgt gcagtacagc gcctgcgcca
     2161 agtacaccaa cggcccaccc ctgaagggcc acatccagag catcctgtgc gcctgcaccg
     2221 actacatctg ggacaaccac ttccaccagc tggacgacga gcagatcgtg cagagcctgc
     2281 tgaccaaccc caagtgcccc agcgacggct acgagacaaa gacccggccc tgcatcatca
     2341 tcaaccagcc cctggccccc aacggctgca ccaacttcaa catctctacc accgagttcg
     2401 ccctgttcag cagcaagtac agcaagtgga accagatctc catcctgtgc acccaggtca
     2461 tcgaggacga caccggctcc aagctgttca actgcatcaa gggcgacccc atcaagatga
     2521 accagctgag cctgcacatc gagctgcctc acgtgtacaa gagcaagctg cccatcaccg
     2581 gcaaagtgaa gctgctgaac tacgacaaga acaccaacta cagcgtgaag atcagcgtgt
     2641 ccgaccagaa gtggggctgc tactggcaga acggcaacac cgacaaccag cactacctga
     2701 atgtgatctc catgaacagc aacggcgagg gcctgatcta cctgcccccc atcagcagcc
     2761 agcacagcat cagcgtggaa gccgagctgc tgcagagcag cgtgtccagc acccggtcca
     2821 gcctgaacag cagcaccagc tccaacgaaa ccaccagcaa caccaccaac cagaacagcc
     2881 cccggaagct gaccaacgag aagcccagca gcctgtacta ctggccccac cggccctgga
     2941 tcaacgatca ccggatcatc gacaacgtgg tgctgcggag ctgggacagc atctcccaga
     3001 tctatctgca gctgtggccc cctcacgaca aagtgatcac cacctgtccc aacctcgtga
     3061 agctgaccct gctgagcaac acccggctga gcgacaaggc catcttcatc gagagcgtga
     3121 tccggggcaa gcaccagaaa atcgtgatcc ctgccgacag catcgacaat gacaacgact
     3181 actgcatcga ccgcgacgac gagctgggcc actacaagtg cctgaactgg aacagcaccg
     3241 agatcgagtg cctgcccggc tggaacggcg agaactgcct gatccccgtg tgcagcctgg
     3301 aatgcagcaa gaagggcggc ttctgcagca agcccaacca gtgccagtgc aagaccggct
     3361 ggaccggcat cacctgtgac cagtgcgtga agagagagaa ttgcctgcac ggcaagtgcc
     3421 tgctgggcaa cgactgcatc tgcgagcctg gctgggccgg ctacctgtgc gacagcaaga
     3481 aagtgatcta cgagaagatc gaggatgaca gcgagacaaa ctccaccgac agcgtgaccg
     3541 aggaaatcaa caacgaggac agcgagaagc acagcaccat gaactccccc agccagatca
     3601 acaccaagcc catccggacc ctgtaccagc ggtacatcca gttcaagatc gacggcagct
     3661 ggggccccaa gttcaccgcc gtgctgtact tccacaatca gcacgacgtg accatccccg
     3721 agatcatcag cacccagatc accatcgagc agattgacaa ctgctccagc aacgccattg
     3781 ccctggaaag caagagcaac cacaccaacc tgaagctgag caagaagatc gccgaccccg
     3841 gcgagcagat caagatgaca atcatccccc agggcgtgtc ccagtggatg gaaaagtcca
     3901 ccagctacga catgaagtcc ctgaactcca acggcgacaa caacaacgac aagggacagc
     3961 tgagcaacga gctgtgcttc gtgcggatct ccgacatcag cctggacaac ttccagggcg
     4021 acaagaacct ggtcaacctg aagtacttta ccgacaagct gattgagttc accggcgaga
     4081 gcggctaccg gcccctggtg gccagctcca cccaggaagc ctttctggcc gctggcttcc
     4141 agctgggcag cacctacccc gagcccacct tcgtgcacag agtgtacccc tgcccctacc
     4201 tggcctacag caacgccctg cccgtggtca tggacgatgc tgtgggcgac ggcgagtaca
     4261 agtccaacag cgagcaccgg cagaccatca gcctgcggaa gggcctgcac gacaccatca
     4321 agccccggct gcgggacttc ttcccagaag tctggctgtt cgacgtgatg cctatcacca
     4381 acctgcagaa caacgacgcc gacgactaca acgagaacga catcgagaac accggcttca
     4441 acgagacaaa caagctgaag gatatcgagc tgaacctgac cgtgcccgat accatcacca
     4501 gctggcgggc cagcgcctac tgcaccacca aagaaaacgg cctgtggatc ggcgagcccc
     4561 agatcctgac cgtgaccatg cccttctaca ccgagatcac cctgcccaaa gagatgaagc
     4621 ggggcgagat cctgcacatc cccatcagcg tgttcgtgct ggaccggaag tacctgaacc
     4681 ctaccgacga caatttcaac aagacagagt gctacgaaac cagcgtgcgg atccaggtca
     4741 acaacagcga gtggctgatc acaaccagca acgagttcac caattgcctg tgctccggcc
     4801 agaaaatcac ctacctcgtg ggcctgctgc ctcagcagct gggccagctc aacgtgaccg
     4861 tggaagcctt cgccatccgg aacagcgagt tctgcggcct gatccagacc aacgacaacg
     4921 gcaacagcct gaccttcgag agcaacaaga ctgagatgaa gaccaaccag tccaagctgc
     4981 tgtccgacaa gatgatccgg cagatcaatg tgatccccga gggcatccag caggaaacca
     5041 ccctgagcga catcgtgtgc ctgaatgagc agcagaccac cagcaccaga cacttccagt
     5101 tcaccctgcc agaccacatc atcaaggaca gcttccacag ctacatcagc tacagcgacg
     5161 aggtgctggg ccctgccctc gtgaacctgg acagcctgat ccggctgcct accggctgtg
     5221 gcgagcagaa catggtgctg gtcgccccca acgtgtacat cctggactac ctgaagtcca
     5281 cccccaacat cggcatcaac gacaacaaag agaagtatat cgagagcgcc aagagccaca
     5341 tcatctccgg ctactaccag cagatgaagt accggcggga cgacggctcc ttcagcgcct
     5401 tcggcaagag cgacaaagag ggctccacct ggctgaccgc cttcgtgctg agagtgttcg
     5461 ccaaggccta cagactgcag cccaacctga gcatcgactg gaacaacctg ttcaacggcg
     5521 ccatcaacta cctgatcatg caccagaaca atgagagcgg ctgcttcgag gaatacggca
     5581 aggtgctgta cagccccctg cagggcatca gcggcaccga cgagacacag tggcggagca
     5641 tcctgctgac aagctacgtg tccagcgccc tgctggaaat ccagagcacc ttcgacaaag
     5701 agaacgacga gaacaaggtc gactacctgc acatcaagaa ctacaccaca gagatcaaga
     5761 atagcttcga cgccgtgttc cggtgcctga ataccaagct ggtgtctctg gacttcttca
     5821 aagaaatccc cacctccgtg ctggtgcagc tgagctacac ctacaccatc atcaagccca
     5881 acaacgagct gaccgtgaag ctgcagaatg agacaatcaa ccggaagcag gtcacaaacg
     5941 accagttcgg caccaagatc ttttggagca gcaactactg cgagaacaac atgaccaccc
     6001 agaagaacaa gaacgagccc cgggacctgg aatccaccgc ctacgccttt ctgatgctga
     6061 accagatgaa tcagtccgtg tccgatctgt tccccatcat ccggtggatc agctcccagc
     6121 agaagtccaa cggcggcttt tacagcaccc aggacaccgt gctgagcctg gaagccattg
     6181 ccgagtttgc caagcggctg ggcctgagca agaccctgga cagcaacgtg tcctccaacg
     6241 tgctgttcat cagcaacaag atgtccttcg tgaactacac cctggacgat ctgatcacca
     6301 gcgagaagag acaggtcatc aatcagattg agctgcccag ataccccctg gacgacaacc
     6361 agaagatcat cgactccacc tgggagctga agtccaatga gagccagacc gactgcatcc
     6421 tggtgcagaa caccttcatc tacaacatgc ccgagagaga ggacatcgac aagaagatca
     6481 agctgagctt cgacctggtg cagcagaaca agctgagcga cgtgaactgc aagctggcca
     6541 ccctgtccat gtgcctgcag ctcaacaaca tgaagaacgt gtccaccatc agcaccggca
     6601 tgctgctgat cagagtgaca atggtcaccg gctgggagcc catcctggaa gaactgcaca
     6661 gccagctggg ctccgaggac gagagcctga gaaagttcac catgaacgac cagaacgaga
     6721 tctccctgta cttcgacgag ttcagcgaga acgaaacccg gaaagtgggc ggcgactgga
     6781 ccaagctgaa gagatgcgtg tccctgcccc tgaagcagat ccactacgtg gaaaacgcca
     6841 agaccgccac catcacaggc ttcgagtact acacccccga cgagtccgtg accgtgacat
     6901 accagctgga tgagtgccgg cagggctgga acatcagcat cgaccacacc accaccgacg
     6961 acttcaccga gtccatcatg accaccgaca cctccatcca gaatatcacc accacccccc
     7021 agatgaagac actgtgcccc atctgcatca accagggcaa tcacaccgcc ctgctgcccg
     7081 aggacatctt caccagcatc tgcaactaca ccaatggcat ctacctcatc aaggtgctcg
     7141 agtacaacaa caataccatg aacgtcacca tcatccagat cctgcagtcc cggtacatca
     7201 cctcttggaa caccaccatc ctgttcccca acctgctgga atgctcctgc aatatcatcc
     7261 agacccagca gaatctggtg ctgttcttcg accagtacac caatatctac cagggcgacg
     7321 cccagatcaa tctgaacacc atcgacacca atgtgaccct gatctccacc aacgacgtgc
     7381 tgcccaatct gaagctggcc aatgagaaat ggctgaccat gagcaacagc accaccgatg
     7441 acgacttcaa ctactacgac ctcttcaact acaactgcaa gcggctgccc ctgatcgtga
     7501 actatatcca ggaaaagttc ctgaacggcg cgccgtcgac ctccatcacg gcctataaga
     7561 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     7621 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     7681 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     7741 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     7801 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     7861 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     7921 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     7981 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     8041 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     8101 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     8161 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     8221 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     8281 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     8341 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     8401 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     8461 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     8521 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     8581 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     8641 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     8701 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     8761 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     8821 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     8881 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     8941 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     9001 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     9061 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     9121 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     9181 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     9241 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     9301 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     9361 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     9421 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     9481 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     9541 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     9601 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     9661 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     9721 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     9781 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     9841 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     9901 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     9961 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
    10021 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
    10081 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    10141 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
    10201 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    10261 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
    10321 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
    10381 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
    10441 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
    10501 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
    10561 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    10621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    10741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    10801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
    10981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    11041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    11101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    11161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
    11281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    11341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    11401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    11461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    11521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    11581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    11641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    11701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11881 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    12001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    12061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    12121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    12541 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    12601 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    12661 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    12721 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
    12781 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
    12841 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
    12901 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
    12961 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
