LOCUS       sequence-229205-        7422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_112090-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2586..2603
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2655..2710
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2709..2728)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4946..4964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5032..5136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5137..5205,5206..5997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5355..5374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6168..6756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6657..6676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6910..6927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7044..7065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7080..7085,7086..7103,7104..7110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7118..7134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7123..7145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7142..7158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7142..7158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ttgagagagc ctctacctgg ctcgtgcgga agggcgagcc cgtgcaggac cggaccgagt
     1261 tcccctatat cgccttcgtg cggaccgagc ggaccatgtg caccggcagc ctggtgtcca
     1321 ccagagccgt gctgacagcc ggccactgcg tgtgcagccc catgcctgtg gtccaggtgt
     1381 ccttcctgac cctgcggaac ggcgaccagc agggcatcca ccaccagccc tctggcgtga
     1441 aggtggcccc cgagtacatg cccagctgca ccgccagccg gcagcggcgg agaatcagac
     1501 agaccctgag cggcttcgat atcgccaccg tgatgctggc ccagatggtc aacctgcaga
     1561 gcggcatcag agtgatcagc ctgccccagg ccagcgacat ccccacccct ggcaccgacg
     1621 tgttcatcgt gggctacggc cgggacgaca acgaccggga cccatctaga cgggctggcg
     1681 gcatcctgaa gaaaggcaga gccacagtga tggaatgcaa gcacagcacc accggcaacc
     1741 ccatctgcgt gcaggccgcc tacgtgttcg gccagatcac cgcccctggc gactctggcg
     1801 gccctctgct gagatctcct cagggccctg tgctgggcgt ggtgtctcac ggcgtgaccc
     1861 tgagcaaccg gctggacgtg ctggtggaat acgccagcgt ggcccggatg ctgggcttcg
     1921 tgtccagcaa cattggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     1981 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     2041 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     2101 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     2161 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     2221 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     2281 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     2341 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     2401 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     2461 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     2521 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     2581 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     2641 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2701 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     2761 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     2821 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     2881 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     2941 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3001 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3061 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3121 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3181 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3241 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3301 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3361 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3421 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     3481 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     3541 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     3601 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     3661 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     3721 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     3781 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     3841 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     3901 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     3961 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4021 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4081 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4141 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4201 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4261 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4321 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4381 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4441 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4501 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4561 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     4621 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     4681 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4741 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     4801 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4861 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     4921 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4981 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5101 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5161 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5221 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5281 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5341 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5401 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5461 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5521 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5581 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5641 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5701 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5761 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5821 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5881 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5941 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6001 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6061 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6301 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6781 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6841 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6901 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6961 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7021 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7081 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7141 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7201 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7261 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     7321 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     7381 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
