LOCUS       sequence-229207-        8118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_002150-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3282..3299
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3351..3406
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3405..3424)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5642..5660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5728..5832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5833..5901,5902..6693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6051..6070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6864..7452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7353..7372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7606..7623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7740..7761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7776..7781,7782..7799,7800..7806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7814..7830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7819..7841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7838..7854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7838..7854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gcgagtacga ctacaacaaa gagtacatcg acgaggaaag cctgaaccag ttcatcaaca
     1261 acagcaacga cggcgacgac gactacgacc acgatcacca ctaccggcat cacgaccacc
     1321 accacagaca ccatgatcat caccacaggc accaccccca gcacatccac aagaacgtgg
     1381 gccggaagat ccagcagaag ttcttcccac tgagcttcca cttcaaccgg cccaagagcc
     1441 aggaaatcta cgtcttcaac atcgagcggt acagcaattg gagcgactgg aaagaatgcc
     1501 tgcccatgga atgcatcgag atccggtaca gaaagtgcct ggacgacagc tggaaaacca
     1561 tcagccccaa cctgatccac accacccggt gcatcagcaa gtactacgcc gagaagcgga
     1621 cctgcctgaa caagacccag tgcaacgagt acaccggcga ccagatcatc aagaacctga
     1681 ccaatacctg cggcatccgg aagtccgaca accagatcat ggaaaagatc ctgggcggca
     1741 aggccgtgga accccactct tggccttggg ccgtgcggct gagcgtgaag ctgcctcggc
     1801 ggagaagcgt gaccttctgc ggcggcacac tgatcgcccc ccagtggatt ctgacagccg
     1861 cccactgcgt gctggtggaa aacaagcaca tccccgtggg caagcccgtg atgctggccg
     1921 accacatgaa gtccaccatc tacgcccacc tgggcgacca cgaccggtac aagcaggaag
     1981 ccgcccagat cgaccacaga gtgaccgtgg ccatcctgca ccccaactac caccggaagc
     2041 tgcagaccga cggctacgat atcgccctgc tgagactgag cgagcccgtg aaaaccaccc
     2101 ccgagatcga cttcgcctgc ctgcccagca agaatctgaa gctgaccagc aactccaagt
     2161 gctacgccgt gggctggggc agcaacaagg gcggcaagat ccccaccttc gacaacatcc
     2221 actctatcct ggaaagcctg ttcctgccct tccccagcct gttcaacacc cccttcacct
     2281 tcggcagaag agagagcagc atctggaaca tcaagaagct ggaagaggaa gagtccagca
     2341 aagaactgca cgaggtggaa ctgcccatcg tgtccatcga cgactgccgg aagtactatg
     2401 ccgacatcag cagcaaggtg cacgtgtgcg ctggcgccaa gaacaaggac acctgtgccg
     2461 gcgatagcgg cggaggcctg tactgctacc tggaagatac caaccggtgg cacatcgtgg
     2521 gcgtgaccag cttcggcctg gccagaggct gtggcctgaa ccccggcgtg tacaccagca
     2581 ccagcagcca catggactgg atcagcaagc agctggccac caagatcttt ggcgcgccgt
     2641 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2701 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2761 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2821 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2881 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2941 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     3001 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3061 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3121 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3181 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     3241 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     3301 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     3361 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3421 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     3481 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     3541 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     3601 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3661 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3721 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3781 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3841 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3901 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3961 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     4021 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     4081 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     4141 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     4201 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     4261 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     4321 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     4381 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     4441 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     4501 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     4561 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     4621 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4681 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4741 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4801 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4861 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4921 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4981 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     5041 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     5101 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     5161 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5221 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     5281 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     5341 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5401 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5461 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5521 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5581 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     5641 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5701 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6061 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6361 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6781 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6841 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6901 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6961 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7021 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7081 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7201 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7261 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7321 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7381 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7441 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7501 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7561 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7621 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7681 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7741 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7801 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7861 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7921 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     7981 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     8041 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     8101 catgtccaat atgaccgc
//
