LOCUS       sequence-229208-        7662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_141610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2826..2843
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2895..2950
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2949..2968)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5186..5204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5272..5376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5377..5445,5446..6237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5595..5614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6408..6996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6897..6916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7150..7167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7284..7305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7320..7325,7326..7343,7344..7350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7358..7374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7363..7385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7382..7398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7382..7398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 acgagatcga cgccagacgg cacaagcgga tgtaccagcc cctgagcatg gaactgatca
     1261 acttcatcaa ctacgaggcc aacaccacct ggaaggccgc tcccaccacc cggttccgga
     1321 ccgtgtccga catcagacgg atgctgggcg ccctgcccga ccccaatggc gagcagctgg
     1381 aaaccctgtg caccggctac atcagcgacg agctgcccaa gagcttcgac gcccgggtgg
     1441 aatggcccca ctgccccagc atcagcgaga tccgggatca gagcagctgc ggctcctgct
     1501 gggcctttgg cgccgtggaa gccatgagcg accggatctg catcaagagc aagggcaagc
     1561 acaagccctt tctgagcgcc gagaacctgg tgtcctgctg cagctcctgc ggcatgggct
     1621 gcaacggcgg ctttcctcac agcgcctggc tgtactggaa gaaccagggc atcgtgaccg
     1681 gcgacctgta caacaccacc aacggctgcc agccctacga gttcccccca tgcgagcacc
     1741 acgtgatcgg ccccctgccc agctgtgacg gcgacgtgga aacccccagc tgcaagacca
     1801 actgccagcc cggctacaac atcccctacg agaaggacaa gtggtacggc gagaaggtgt
     1861 accggatcca cagcaacccc gaggccatca tgctggaact gatgcggaac ggccccgtgg
     1921 aagtggactt cgaggtgtac gccgacttcc ccaactacaa gagcggcgtg taccagcacg
     1981 tgtcaggcgc cctgctggga ggacacgccg tcagactgct gggctggggc gaagagaaca
     2041 acgtgcccta ctggctgatc gccaacagct ggaacagcga ctggggcgac aagggctact
     2101 tcaagatcgt gcggggcaag aacgagtgcg gcatcgagag cgacgtgaac gccggcatcc
     2161 ccaagatcaa gaatggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     2221 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     2281 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     2341 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     2401 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     2461 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     2521 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     2581 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     2641 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     2701 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     2761 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     2821 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     2881 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2941 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3001 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3061 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     3121 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     3181 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3241 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3301 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3361 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3421 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3481 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3541 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3601 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3661 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     3721 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     3781 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     3841 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     3901 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     3961 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4021 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4081 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     4141 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     4201 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4261 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4321 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4381 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4441 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4501 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4561 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4621 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4681 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4741 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4801 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     4861 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     4921 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4981 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     5041 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5101 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     5161 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5221 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5281 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5341 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5401 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5461 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5521 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5581 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5641 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5701 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5761 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5821 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5881 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5941 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6001 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6061 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6121 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6181 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6241 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6541 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7081 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7141 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7201 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7381 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7441 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7501 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     7561 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     7621 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
