LOCUS       sequence-229211-        7917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_075800-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3081..3098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3150..3205
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3204..3223)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5441..5459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5527..5631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5632..5700,5701..6492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5850..5869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6663..7251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7152..7171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7405..7422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7539..7560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7575..7580,7581..7598,7599..7605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7613..7629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7618..7640
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7637..7653
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7637..7653
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 agctggacac caactacgag gtgtccgacg agacagtgtc cgacaacaac aagtgggccg
     1261 tgctggtggc cggcagcaac ggctacttca actaccggca ccaggccgac gtgtgccacg
     1321 cctaccacgt gctgcggagc aagggcatca agcccgagca catcatcacc atgatgtacg
     1381 acgatatcgc ctacaacctg atgaacccat tccccggcaa gctgttcaac gactacaacc
     1441 acaaggactg gtacaagggc gtggtcatcg actaccgggg caagaaagtg aacagcaaga
     1501 ccttcctgaa ggtgctgaag ggcgacaaga gcgctggcgg caaagtgctg aagtccggca
     1561 agaacgacga cgtgttcatc tacttcaccg accacggcgc tcccggcctg atcgccttcc
     1621 ccgatgacga gctgtacgcc aaagaattca tgagcaccct gaagtacctg cacagccaca
     1681 agcggtacag caagctggtc atctacatcg aggcctgcga gagcggcagc atgttccagc
     1741 ggatcctgcc cagcaacctg agcatctacg ccaccaccgc cgccagcccc acagagagca
     1801 gctacggcac cttctgcgac gaccccacca tcaccacctg tctggccgac ctgtacagct
     1861 acgactggat cgtggacagc cagacccacc acctgaccca gcggaccctg gaccagcagt
     1921 acaaagaagt gaagcgcgag acaaacctga gccacgtgca gagatacggc gacacccgga
     1981 tgggcaagct gcacgtgtcc gagttccagg gcagccggga caagagcagc accgagaacg
     2041 acgagccccc catgaagccc cggcactcta tcgccagcag agacatcccc ctgcacaccc
     2101 tgcaccggca gatcatgatg accaacaacg ccgaggacaa gtccttcctg atgcagatcc
     2161 tgggcctgaa gctgaagcgg cgggacctga tcgaggacac catgaagctg atcgtgaaag
     2221 tgatgaacaa cgaggaaatc cccaacacca aggccaccat cgaccagacc ctggactgca
     2281 ccgagagcgt gtacgagcag ttcaagagca agtgcttcac cctgcagcag gcccccgaag
     2341 tgggcggcca cttcagcacc ctgtacaact actgcgccga cggctacacc gccgagacaa
     2401 tcaacgaggc catcatcaag atctgcggag gcgcgccgtc gacctccatc acggcctata
     2461 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2521 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2581 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     2641 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     2701 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     2761 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     2821 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     2881 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     2941 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     3001 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     3061 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     3121 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3181 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3241 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     3301 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     3361 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3421 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3481 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3541 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3601 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     3661 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     3721 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     3781 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     3841 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     3901 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     3961 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4021 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4081 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4141 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4201 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4261 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     4321 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     4381 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4441 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4501 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4561 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4621 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     4681 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     4741 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     4801 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4861 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4921 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4981 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5041 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5101 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5161 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     5221 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     5281 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5341 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     5401 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5461 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5521 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5581 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5641 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5701 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5761 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5821 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5881 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5941 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6001 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6061 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6121 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6181 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6241 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6301 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6361 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6421 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6481 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6541 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6601 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6781 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7381 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7441 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7501 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7561 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7621 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     7681 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     7741 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     7801 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     7861 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
