LOCUS       sequence-229213-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_132480-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2985..3002
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3054..3109
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3108..3127)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5345..5363)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5431..5535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5536..5604,5605..6396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5754..5773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6567..7155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7056..7075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7309..7326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7443..7464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7479..7484,7485..7502,7503..7509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7517..7533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7522..7544
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7541..7557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7541..7557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agatcgtgaa gatccccctg caccccctgg aaaagagcga cagcctgctg gaacactaca
     1261 ccagctactt ccggcccagc aagcggaagt ccatgtacca gtggaacaag cagaacacca
     1321 gcacccccga gcagctgatc aacttcaaga acctgcagta ctacggcgag atcagcgtgg
     1381 gcaccccccc tcagaaactg cgggtgctgt tcagcaccgg cagcatcgac acttggtttg
     1441 ccagcagaaa gtgctggttc ctggacatct tctgctggat gttccggttc tacgacagca
     1501 gcaagagcct gacctacgtg gccgacggca gcagcttcca cgtgtcctac ctggacgaca
     1561 gcctgagcgg cttttggagc gtggacacca tccggatcaa cagcctggtc atccccaacc
     1621 aggccttcgg cgagatgcgg aacatcttca gcctggacta cttcacctct aagtacgacg
     1681 gcatcatcgg catgagcagc agccggatca gcaagttcgg caagatcccc atgttcccca
     1741 acctgctggc caacggcgtg aacatggacc ccatcttcag cttctacctg aaccaggaaa
     1801 atggcgcccc tatcggcggc gagatggtgc tgggcggcgt gaaccctgag tacttcgagg
     1861 gcgacttcga gtacatcccc accgtgcaca acaacatgtg ggccgtgggc atgtccagcc
     1921 tgatgatcaa gggcgtggaa ttctgcaacg gctgcaccgc ctccatcgac accggcacct
     1981 ctgtgatcct gggcgagaga gagcaggtca accggatcaa ctccctgctg ggcgtgtcct
     2041 atcacgtgat cgagcggaac gtgctggact gcagcagaat cgacatgctg ccccccatcg
     2101 agttcatctt ccagaagaga aagtacatcc tgctgcccca gcactacgtg gtcaaggtgt
     2161 acctgtggtt cggcaccatg tgcagcagcc ccttcgaggc cgacgactcc ctgcccagca
     2221 acgtgtggat cctgggggac gccttcatgc ggcggttcta caccgtgttc gacttcggcc
     2281 agaagcggat cggcctgaca tacgtgtcca acaagaagaa cggcacccgc tctggcgcgc
     2341 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2401 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2461 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2521 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2581 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2641 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2701 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2761 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2821 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2881 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2941 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     3001 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     3061 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3121 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3181 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3241 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3301 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3361 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3421 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3481 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3541 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3601 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3661 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3721 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3781 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3841 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3901 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3961 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     4021 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     4081 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     4141 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4201 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4261 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4321 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4381 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4441 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4501 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4561 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4621 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4681 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4741 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4801 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4861 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4921 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4981 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     5041 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5101 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5161 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5221 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5281 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5341 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5401 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5461 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5521 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5581 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5641 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5701 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5761 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5821 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5881 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5941 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6001 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6061 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6121 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6181 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6241 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6301 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6361 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6421 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6481 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7201 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7261 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7321 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7381 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7441 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7501 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7561 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7621 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7681 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7741 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7801 gctcatgtcc aatatgaccg c
//
