LOCUS       sequence-229214-        7641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_166280-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2805..2822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2874..2929
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2928..2947)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5165..5183)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5251..5355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5356..5424,5425..6216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5574..5593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6387..6975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6876..6895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7129..7146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7263..7284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7299..7304,7305..7322,7323..7329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7337..7353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7342..7364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7361..7377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7361..7377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 gcttcaccag cagcttcacc aagggcaaca tcatccagcc cgagctgagc cccgactacc
     1261 tgttcacccg gctgcggagc agaagcgtga agaacagcct ggaacggatc ttcaacgaga
     1321 tcaatatcat ccggaacgtg ggcacccctg gccacctgaa agtgcgcgag ttcatccagg
     1381 actacctgaa caagcggggc tgggagacag acctggacac cttcgaagag aacaccgtcg
     1441 tgggcgtgaa aaccttccac aacatcatcg gctacagaag cgaccccaac tacaccaagt
     1501 acctgatcct ggcctgccac tacgagagca agctgctgcc caacttcgtg ggcagcaccg
     1561 acagcgccat gccctgcagc atcatcctga agatcgtgga catgctgaac tacggcatca
     1621 aggccctgaa gaagtccgac atcggcctga agctgatctt cttcgacggc gaagaggcct
     1681 tcaaccagtg gaccgacacc gacagcctgt acggcagccg gcacctggcc aagaagtgga
     1741 acagcaccac cttcaagagc agcaacgaga cagagctgag caagatcgac ctgctggtgc
     1801 tgctggacct gctgggcgcc aagcagcccc acatccccaa gtacccctac gaggacaagg
     1861 gcagcttcaa catgctgatc gagctggaac ggcggatgcg gtccttcaac ctgctgaagt
     1921 ccagcagcga gaacaacaag ccctacttcg gccacgccgt gcactaccag atcggcgacg
     1981 accacatgcc cttcgtggaa aagggcgtgc ccgtgctgca cctgatcccc ctgcccttcc
     2041 caaaagtgtg gcacacaatc gccgacaacg ccaccatcat cgactgggac acctccgtgg
     2101 atctgctgct gctgattgag ctgttcgtgc ggaactacct gcacatcctg ctgggcgcgc
     2161 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2221 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2281 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2341 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2401 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2461 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2521 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2581 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2641 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2701 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2761 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     2821 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2881 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2941 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3001 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3061 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3121 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3181 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3241 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3301 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3361 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3421 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3481 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3541 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3601 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3661 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3721 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3781 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3841 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3901 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3961 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4021 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4081 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4141 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4201 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4261 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4321 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4381 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4441 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4501 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4561 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4621 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4681 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4741 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4801 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4861 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4921 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4981 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5041 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5101 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5161 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5221 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5581 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5881 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6001 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6241 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6301 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6361 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6421 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6481 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6541 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6601 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6661 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6721 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6781 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6841 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6901 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6961 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7021 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7081 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7141 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7201 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7261 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7321 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7381 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7441 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7501 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7561 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7621 gctcatgtcc aatatgaccg c
//
