LOCUS       sequence-229215-        7653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_187140-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2817..2834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2886..2941
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2940..2959)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5177..5195)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5263..5367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5368..5436,5437..6228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5586..5605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6399..6987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6888..6907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7141..7158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7275..7296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7311..7316,7317..7334,7335..7341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7349..7365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7354..7376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7373..7389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7373..7389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agcggaccct gaagttcaac gacctgtggg agaagtggaa gtccgtgtac accaagcagt
     1261 acaccgacga ggacgaggaa gccatccggc ggagaatctg gaacaagaac ctgatcaaga
     1321 tccagctgca caacctgaga tacgacctgg gcttcgagac atacagcatc ggcctgagcc
     1381 agttcaccga catcgagtgg aacgagttct acagcatgta caaagtgaaa gagcggctga
     1441 tcatccccga gagcagcccc agcagcagct tcatcgaaga ggactacgac gtgaacaaca
     1501 tcggctggac ccccgacagc tacgactggc ggcacttcaa catcgtgaac gagccccggg
     1561 acgagggcag ctgcatcggc agctacgcct tcgccgtgac agccgtgacc gagagccagt
     1621 acgccctgcg gaccagcaac cggaagaaat tcagcgtgca gcagtttatc gactgcaccc
     1681 ggatctacgg caacatgggc tgccacggcg gctacacctt caccctgttc atctacctgc
     1741 agaacttcgg cctggaaacc gaggaaaact accccttcac tggcggcgac caggactgcg
     1801 tggccaacag ctccgacgtg gtggtgcaga gcatcggcta caagttccac cggcacggct
     1861 acgagacaat cctgaagtgg gccgtgtaca acgagggccc ctacgtgatc agcatgaata
     1921 tcgacgagaa cttcctgcac tacaagagcg gcatctacca gagcgatacc tgcacccact
     1981 acaacctgaa ccagagcatg ctgctcgtgg gctacggcta cgacaacgac ggcaacgact
     2041 actggaccct gcagaacaac tggggcacaa agtggggcga gcagggctac gtgaaggtgc
     2101 tgcggaacca ctggaacatg tgcggaatcg ccagcatggc cttccggccc atcctgcggg
     2161 gctttggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2221 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2281 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     2341 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     2401 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     2461 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     2521 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     2581 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     2641 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     2701 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     2761 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     2821 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     2881 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     2941 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     3001 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     3061 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3121 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3181 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3241 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3301 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     3361 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3421 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     3481 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     3541 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     3601 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     3661 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     3721 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     3781 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     3841 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     3901 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     3961 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     4021 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     4081 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4141 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4201 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4261 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4321 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     4381 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4441 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     4501 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     4561 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     4621 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4681 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4741 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4801 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     4861 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     4921 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4981 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     5041 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5101 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     5161 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5221 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5581 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5881 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     5941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7141 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7201 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7261 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7321 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7381 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     7441 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     7501 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     7561 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     7621 acatttatat tggctcatgt ccaatatgac cgc
//
