LOCUS       sequence-229217-        8385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_090110-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3618..3673
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3672..3691)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5909..5927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5995..6099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6100..6168,6169..6960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6318..6337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7131..7719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7620..7639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7873..7890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8007..8028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8043..8048,8049..8066,8067..8073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8081..8097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8086..8108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8105..8121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8105..8121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agtatatctg ccagctgatg cccaacatca caatggtgtc catgatcgtg ttcaagaccc
     1261 ggatgagcct gttcaagaac ttcggcatcc tgggcgagcc catcgacaag agacagtacg
     1321 gcggcaaccg gctgaagttc atccccatct acagccggaa catggaaatg caccccctgt
     1381 tcagcagatt caagaccgag gtggtggccg tggccctggc cttttgggag aaagctctga
     1441 gcgtgcggaa gccccccaac cggaagctgc tgattgagcg gggctgcgtg gaacccagct
     1501 acttcagaga cagaagcacc ggcaagctgt tctgcaagag ccagtgcaga cccagagccc
     1561 tgtgctacga tcacgaggtg cccaacgagt acgccagcgg atgtgctgtg ggcacaggca
     1621 gcggctacat ccgggacgtg taccaggatg gctccggctt cgcccccaat gagtacgtgc
     1681 tgttcatcag cagcgacaac aagaagggct gcctgagcgg cgtgacactg gcctatgccg
     1741 gaccttgcga gatgcacccc accaccgaca gacccattat gggcgctatc aacttctgtc
     1801 cacaaaagat ggaaatcgag gaacccggca agaccatgct gatcggcacc gccattcacg
     1861 agctgggcca cgccatgcag atcaagatca gctatatctt catcttccgg tttctgaact
     1921 tcttcttcgt ggtgttccac ttcagcgaga acaccgtgaa gcggatcgtg cggccttggg
     1981 tcacagccgt gggcagcttc atcaagccct tcagcagcat cgtgacccct gccctgctgg
     2041 acgagggccg gaagcactac aactgcccca acctggacgg catcgacctg gaaaatgagg
     2101 gcggcgaggg cacagtgggc agccactttg agaagcgggt cgtgggcgac gagatcatgg
     2161 ctggcgtgac cggcgtgaaa accgtggtgt ccagactgac cctggcattc ttccaggaca
     2221 gcgggtggtg ggacgtgaac tacagcgtgg ccgaggactg gaactacggc aagaacctgg
     2281 gctgcacctt cgtgcgcgag agctgctacg cctacatgat gaagatgaag ctggaaggca
     2341 agagcatgga accctactgc gaagaggccg acagcctgat gtgctaccac cacaaggcct
     2401 tcggcacctg tgccatcggc cagtacatga agtacctgca gccccaggaa cagtacttca
     2461 agggcgctcc cgactatggc ggaacaggcg ccctggtgga taagtgccct ctgatccagc
     2521 ccaccaagca gttctttgag gaacccctga tgacctactg tgaccacagc ctgaacatcc
     2581 ctgtggccca gagaggcaac atcttcggcc aggcctttgg caacaacagc gtgtgcatca
     2641 cccacaaggg ggcctggaag gccagactga atggcaaggc cacccaggac ccccggatca
     2701 aggccacatg tcaccagtac agctgcagcg gcggagtgca agtgatcatc gacggcaaga
     2761 gcttcagatg cgacaacggc gtggccaaga tccacaccga gagaatcgag ggcgaggcca
     2821 tctgccccaa tcccaacgac gtgtgcagag tgcgcatcgt gtacgtggaa cggaaggccg
     2881 tggactgcga gtgcattggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2941 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     3001 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     3061 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     3121 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     3181 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     3241 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     3301 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     3361 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     3421 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     3481 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     3541 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     3601 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3661 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3721 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3781 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3841 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3901 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3961 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     4021 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     4081 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     4141 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     4201 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     4261 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     4321 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     4381 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     4441 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     4501 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     4561 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     4621 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     4681 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     4741 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4801 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4861 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4921 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4981 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     5041 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     5101 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     5161 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     5221 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     5281 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     5341 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5401 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5461 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5521 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     5581 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     5641 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     5701 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     5761 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5821 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5881 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5941 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6001 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6061 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6121 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6181 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6241 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6301 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     6361 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6421 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6481 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6541 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6601 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6661 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6721 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6781 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6841 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6901 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6961 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7021 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7081 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7141 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7201 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7261 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7321 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7381 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7441 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7501 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7561 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7621 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7681 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7741 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7801 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7861 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7921 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7981 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8041 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8101 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     8161 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     8221 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     8281 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     8341 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
