LOCUS       sequence-229218-        7620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_145920-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2784..2801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2853..2908
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2907..2926)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5144..5162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5230..5334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5335..5403,5404..6195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5553..5572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6366..6954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6855..6874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7108..7125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7278..7283,7284..7301,7302..7308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7321..7343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7340..7356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7340..7356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 atcacatcct gatcggcgtg tacaccgtga acgagcccga cgtgaactgc atcagcaaca
     1261 agccccagca gatcgtgttc atcggcggct acccccgcag cggcaccacc ctgatgagag
     1321 tgctgctgga cgtgcacccc atgatcagat gcggccctga gacaagagtg atccccaaga
     1381 tcctgaacct gcggggccag tggtccaagg gcaaagagag cgagcggctg aaggccgctg
     1441 gcctgtaccc tgtggccgtg gatagcgccg tgcggagctt catcagcagc ctgatcaaga
     1501 acgccggcga gaacgccccc gtgctgtgca acaaggaccc cctgagcttc atccacctgg
     1561 aatacctggc cgagatcttc cccgaggcca agttcgtgca catggtccga gatggcagag
     1621 ccgtgatcaa cagcctggtc aagcggaagg tgtccatcaa gggcgagccc aagctgatcg
     1681 acgagcggtt cttcgcctgg gagaacagag tgggccagat gctgaagtcc tgtgccgccg
     1741 tgggccccaa gcggtgcacc accattaagt acgagagcct ggtgctgcgg cccagcgagt
     1801 tcctgcacga gctgttcgag tttctggaaa tcccctggga ccccatcgtg ctgcaccacc
     1861 agaccgtgat cagcgacacc agcctgaaca agctggaacc cagcaccagc caggtggtgc
     1921 accctatcca cctggccagc ctgaccagct gggccagcaa tggcagcgtg ctgcccgaga
     1981 gcttcatctc cagcatccac cgcgagagcg acgtgctgaa aaccctgggc tacgccgacc
     2041 tgggcgtgcc ccctgattac ggcacccccg agaaccaggt ggtcaacatg accagccacc
     2101 tgatcaacga cccccagttc cggcggatct ttggcgcgcc gtcgacctcc atcacggcct
     2161 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2221 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2281 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2341 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2401 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2461 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     2521 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     2581 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     2641 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     2701 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     2761 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     2821 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     2881 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2941 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     3001 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     3061 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3121 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3181 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3241 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     3301 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3361 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3421 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     3481 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     3541 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     3601 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     3661 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     3721 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     3781 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     3841 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     3901 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     3961 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     4021 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     4081 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4141 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4201 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4261 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     4321 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4381 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4441 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     4501 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     4561 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4621 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4681 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4741 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     4801 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     4861 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     4921 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4981 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5041 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     5101 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5161 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     5881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6481 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7381 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7441 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     7501 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     7561 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
