LOCUS       sequence-229221-        7260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_021730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2424..2441
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2493..2548
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2547..2566)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4784..4802)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4870..4974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4975..5043,5044..5835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5193..5212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6006..6594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6495..6514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6748..6765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6882..6903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6918..6923,6924..6941,6942..6948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6956..6972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6961..6983
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6980..6996
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6980..6996
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 gcggaatcaa ggaccggatg ctgaagttca tcaccgacaa ccggaacctg ttcgacaccg
     1261 tggtgtccgg cgacctgaac gtgcccgtgg aaagcaagct gagctggctg caggaagatc
     1321 tgatcgccga gatcagcgag gtggaacccg acaccagcag aggcgagatc aagagcagca
     1381 ccaaagaaaa ccccaacctg gtggccagca gcaacgtgcg ggccgtgcag cagtgccact
     1441 gcagccctca cgaccaccac ctgaagtccc tggtgctgta cagaaacctg cccgccagat
     1501 gcttctgcgg ccactggttt ctgctggtgg acgagcagag atacgaagag gaacgcgaga
     1561 agaagtggaa caagatcaag gacaagcccg agaacgccga cctgctgaag agattcgaca
     1621 tcctggaaaa agagctgaac atcctgctgg ccgagggcaa gagcatgacc cccaagagcc
     1681 ctggcgccga cgagatgatg gacaacatgg ccctgaagtg gaaagagatg aaggaagtct
     1741 acgccaacat ccggaagaac atgctgctgg atggcgcgcc gtcgacctcc atcacggcct
     1801 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     1861 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     1921 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     1981 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2041 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2101 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     2161 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     2221 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     2281 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     2341 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     2401 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     2461 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     2521 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2581 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     2641 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     2701 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     2761 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     2821 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     2881 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     2941 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3001 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3061 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     3121 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     3181 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     3241 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     3301 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     3361 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     3421 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     3481 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     3541 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     3601 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     3661 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     3721 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     3781 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     3841 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     3901 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     3961 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4021 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4081 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     4141 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     4201 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4261 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4321 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4381 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     4441 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     4501 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     4561 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4621 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4681 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     4741 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     4801 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     5521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5881 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6781 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6841 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6901 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6961 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7021 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7081 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     7141 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     7201 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
