LOCUS       sequence-229223-        7104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_007450-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2268..2285
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2337..2392
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2391..2410)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4628..4646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4714..4818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4819..4887,4888..5679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5037..5056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5850..6438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6339..6358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6592..6609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6726..6747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6762..6767,6768..6785,6786..6792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6800..6816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6805..6827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6824..6840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6824..6840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gagtggccgc tcctggcctg tacgtgaaga acagcagcat cgtgctgagc cggtggttca
     1261 ccagcgagaa gtccgtggac atcgacgtga agcggctgaa agaaatgatg aagtccaagg
     1321 acctgaagct gatcgacgtg cgcgagcccg acgagctgga acagaccggc aagatcccct
     1381 acgccgtgaa catccccctg ggcgaggtgg aagatgcctt caagctggac gagaagtcct
     1441 tcctggaaaa gtacaagttc cagaaaccca acgtggccga caagaacgtg gtgttcagct
     1501 gccgcagcgg cgtgcggagc atgcgggccc tgaagatcgt gcaggacctg ggctacgaga
     1561 accccctgaa cctcgtgggc ggctacaacg cctgggccaa agagaatgcc ggcggaggcg
     1621 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     1681 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     1741 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     1801 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     1861 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     1921 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     1981 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2041 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2101 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2161 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     2221 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     2281 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     2341 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     2401 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     2461 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     2521 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     2581 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     2641 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     2701 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     2761 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     2821 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     2881 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     2941 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3001 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3061 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3121 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3181 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     3241 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     3301 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     3361 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     3421 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     3481 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     3541 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     3601 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     3661 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     3721 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     3781 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     3841 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     3901 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     3961 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4021 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4081 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4141 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4201 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     4261 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     4321 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     4381 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4441 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     4501 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     4561 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     4621 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4681 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4741 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4801 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4861 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4921 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4981 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5041 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5101 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5161 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5221 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5281 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5341 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     5401 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5461 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5521 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5581 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5641 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5701 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5761 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6601 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6661 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6721 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6781 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6841 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     6901 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     6961 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7021 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7081 ttggctcatg tccaatatga ccgc
//
