LOCUS       sequence-229225-        7431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_059910-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2595..2612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2664..2719
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2718..2737)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4955..4973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5041..5145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5146..5214,5215..6006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5364..5383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6177..6765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6666..6685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6919..6936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7053..7074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7089..7094,7095..7112,7113..7119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7127..7143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7132..7154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7151..7167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7151..7167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctgattggag cagaggcgac cagaagctgc cattcttcga ccagctgaag tccctggcca
     1261 acaccttcaa caagatcttc cccagcatcc tgagcgccag agccatctgc agcgagaacg
     1321 tgtccatcca gccctgggac tgggattggg acggccggaa ccccagcaac ttccggaccg
     1381 atagcagcct gcagggcgag atcgtgaccc ccaagtgcac ccggcatctg ctgttcatcc
     1441 ggcacggcca gtactactac gccaaagagg acagcgactg ccacctgacc ggcctgggca
     1501 gacagcagct ggactgcacc ggcaagcggc tgagagagct gaacttcccc taccggaagc
     1561 tgtactacag caccatgacc agagccgtgg aaagcgccga gctggtgctg aaccatctgc
     1621 ccaacgtgca ggccgagccc agcgacgcca ttagagaggg cgctccctac atcctggaac
     1681 cccccctggc ctactacaag cccaccccca aggacatcga agaggacggc agccggatcg
     1741 agctggcctt ccggcggcac atccacagag ccgatgtggg ccaggaagcc gacaccgccc
     1801 tgcagtttcc cccagaggcc tggatccggt tcagcctgga ccacggcagc atcacctggc
     1861 tggtcatccg gcccgacggc agagtgaccc tgagatggct gggcaacagc ggccacatgc
     1921 cccccgagct gatcagcgtg cagggcgcgc cgtcgacctc catcacggcc tataagagtg
     1981 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2041 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2101 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2161 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2221 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2281 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     2341 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2401 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     2461 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     2521 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     2581 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     2641 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2701 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     2761 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     2821 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     2881 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     2941 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3001 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3061 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3121 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3181 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3241 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3301 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3361 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3421 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3481 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3541 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3601 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3661 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3721 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     3781 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     3841 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     3901 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     3961 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4021 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4081 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4141 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4201 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4261 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4321 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4381 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4441 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4501 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4561 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     4621 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     4681 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4741 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     4801 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4861 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     4921 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     4981 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     5701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7021 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7081 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7141 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7201 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7261 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     7321 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     7381 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
