LOCUS       sequence-229226-        8055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_089240-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3219..3236
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3288..3343
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3342..3361)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5579..5597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5665..5769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5770..5838,5839..6630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5988..6007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6801..7389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7290..7309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7543..7560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7677..7698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7713..7718,7719..7736,7737..7743)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7751..7767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7756..7778
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7775..7791
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7775..7791
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agttccggcg gtacagcgag ttcagcgagt acgaccacag caacgcctac tggctgtgga
     1261 aggacgtgat cctgagcccc accgacagcc tgtggctgga acccgtgcat ctgcagcacc
     1321 ccaagaacat cagattcgtg atcaacaaga gcagcgagac aagcggccct ctgagctacc
     1381 tgctgccctt cagcagcatc agcaacctgc ggcagtacat cagccacatc aacatggtgc
     1441 agtacgtgaa gaacgaggac ctgtacggcc agctgcccac ccgggacaac ttcaacagca
     1501 cccggtccag ccccaaccac gtgatcatca tccaggtgca caaccggtcc atcgagctga
     1561 gcctgctgat cgagagcctg cggcggacca agggcatcga gacagccctg atcatcttca
     1621 gccacgactt ctacagcgac gagctgaaca gcctgatcgg cagcatccgg ttcacccgga
     1681 ccgtgcagat cttctacccc cacagcatgc agatctttcc cgacaccttc cccggcaccg
     1741 acccccggga ctgcgacagc agaatcaagc ccgccgaggc ccggaacatc gagtgcctga
     1801 acgcccggtg gcccgatacc ttccagcact acagagagag ccacttcacc cagatcaaga
     1861 accactggct ctggaagatc gagttcgtga tgaaccactt ctaccccacc aagtactacg
     1921 agggctactt catcctgctg gaagaggacc acttcgtggt ggaagatatc ctgcacgtgt
     1981 ccgagctgat cagcaagaac gtgtggtccc ccctgaaaac cgacggcatc attgccctgg
     2041 gcagctacga caaggacaac aactacctga gcaacgtggt ggaactgaca tactggctgg
     2101 cccccaagca caacatgggc atggccgtgt cccgggtcgt gtggaagaac atcgagcagt
     2161 gcctgaatct gttctgcttc tacgacgact acaactggga ctggtccctg cagtatatcg
     2221 gccagaactg cttccctggc aagctgaagg ccctgaccct gcccctgagc accagagtgt
     2281 tccacagcgg cgagtgcaga ggcctgcacc accagaaaac cagctgcagc accgagctgc
     2341 tggtcaccaa catcctgcag atgttcagcg gcccactgct gacccggctg taccccacaa
     2401 gcctgcacct gagcgagaag atccacaccg agaacatcaa gcagcgcgtg aacggcgggt
     2461 ggtccgacga gagggacaga tccctgtgcc agagcctgtt cagcaacaag tggaacaaga
     2521 tcctgaccca ctgggcccct aagctgatcg agtacagccc catctatggc gcgccgtcga
     2581 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     2641 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     2701 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     2761 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     2821 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     2881 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     2941 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     3001 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     3061 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     3121 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     3181 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     3241 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     3301 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3361 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     3421 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     3481 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     3541 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3601 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3661 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3721 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3781 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     3841 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3901 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3961 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     4021 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     4081 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     4141 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     4201 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     4261 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     4321 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     4381 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     4441 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     4501 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     4561 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4621 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4681 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4741 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4801 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     4861 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4921 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4981 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     5041 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     5101 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5161 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     5221 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     5281 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5341 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5401 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     5461 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     5521 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     5581 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5761 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5821 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5881 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5941 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6001 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6061 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6121 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6181 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6241 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6301 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6361 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6421 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6481 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6541 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6601 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6661 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6721 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6781 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6841 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6901 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6961 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7021 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7081 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7141 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7441 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7501 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7561 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7621 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7681 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7741 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7801 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     7861 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     7921 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     7981 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     8041 gtccaatatg accgc
//
