LOCUS       sequence-229227-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_134800-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4806..4823
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4875..4930
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4929..4948)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7166..7184)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7252..7356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7357..7425,7426..8217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7575..7594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8388..8976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8877..8896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9130..9147
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9264..9285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9300..9305,9306..9323,9324..9330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9338..9354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9343..9365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9362..9378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9362..9378
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agaccagccc caacgtgtcc ttctgcgagc tgaagcagaa cgagcaccac caccagtacg
     1261 acatcctgat caacaacacc atcatcgagc tgagctgcag cttcggcaac gagtggtaca
     1321 tccccagcac catccccctg aacttcaagc ccatcgtgat catggaccag attaacaaca
     1381 accagagcaa gctgctgatc cagctgccca agaacaacac cgacaacctg cggacccact
     1441 tccggggcct gtaccagtgc tgccagagca gcaacagcta caacatcagc atcatcaact
     1501 ggctgcccaa cgagcccagc tgctgcccct gctgtccttg gtacgagcag gaccagtgcc
     1561 tgttcaagca gatccagttc atcaaccaca gcaacatcat cttcaagctg aagtccatga
     1621 agacctactg cggcagcgtg ttcctgcaca tgaacagcgt gaccatgacc accatccacc
     1681 tggacatcat ccggaaccag cccatgctga tcccttgccc tggcctgaac agcacctacc
     1741 aggaagatag cagcctgttc agcatcgact accagctgac cccccacaag agcagctacc
     1801 agctgtggta tcccttcaag cagttcaagc acagcatcca gtactactac gacaacagat
     1861 tcggcctgtg cctgctgaag tcccagttcc cccggatcat gctgtacatg ctgtgcaact
     1921 acaagcagat gaacgagcag cggatcatcc agcccatctg gcccctgcag agcctggtca
     1981 tcagccccat catccacatc tacctgcaga gcatcaaaga gatcgagtac accacctaca
     2041 agctcatgaa tatcagcagc cacagcttcc tcaacaacat caattggctg atcaagtaca
     2101 gcaacgaggt gctgatcttc cggatcaagc agcacaccaa gctgctgcgg ctgatctgca
     2161 tcggcagcta cacccagaag tacatcgtcc acaagcccat cagcaagctg tgcttccggt
     2221 ggaagatcct gaaccaggac aacgtgaagc tgagcaacat cagcgacttc caggaacact
     2281 accagatcga cgagcagagc ccctgggaga gaaccgagtg cctgaccacc caccagacct
     2341 tcaacaagtt cagcatcctg tacgcctact ctaagtacga cctggacctg agcaagacca
     2401 gcaacgagac aaacagcagc gtgaacaata ccttcatcct gtgcgaagtg accacccccg
     2461 accggatggc ccccatcagc cagagaatca tccagatcgt gaaaaccacc aaccagatca
     2521 gcaacaagct gcacatctcc taccactgca actgcatggt gctgggcgac aacaagtcca
     2581 gcttcagcga caacagcacc ctgagcctgc tgatcaagaa caactccgtg aacctgaaca
     2641 tcagcttttg gaccgagcac gagacagaca accagccctt ctgcatcctg atgaagcacg
     2701 gcatgcccaa gagcgagcag aaaacaaaag agacacagat caaccccatc cggaccaagg
     2761 acacctggaa gtgcttcatc aagatcaaga tgaacctgcc caaccagacc atgaccctga
     2821 gcatcggcta cggcgagatc aagcggacct tccagatctt catcgtgaag tccatcctgg
     2881 aactgacccg gaacgtggaa aacctgcagt cccagatcac ccagcagagc tacctgcact
     2941 gcatccccaa cgacatggaa cggtctatcg cccagagatt cggctaccac taccccaacc
     3001 acgtgacctg gttcatcagc atggacaacc acatccagaa ctacaccaca tacaacaaga
     3061 ccttcgagca catctttaag ctgatcccat tctacaacgt gaccaactac cccatgatcg
     3121 tgctgaacca gaccggcaac ctgtgggagc gccccaacga gctgggccac ctgaacaacc
     3181 tgtacctgaa cgactggccc aagcagctgt gcgtgcggtg cagcagcgtg tacgagatcg
     3241 gcatccactt caacagccac gagagctgca ccctgctgaa cgacttcaac gtgatcgaca
     3301 tcttcaacaa taccggcaac caccgggatc tgcagtccat ccggggcaag aaacagctgt
     3361 acttcgtgat cgagctgaac aacagcagcc agttctacga agtgaacagc cactacaaca
     3421 acagcgagat cagcctggtg cagagcacct tcatcagcct ggaatgcatc agaaagctgt
     3481 acaccaagcc cgagcagttt aagcggaaag agtggcccga gctgaccatt aacaataccc
     3541 agaacaatac cgaggaccac ctcatccaca acaccagctg gatcctgagc ccctacagct
     3601 tcaaagtgaa gaaagtgatc cacctcaaca agcccctggt catccacctg aagtgcgcct
     3661 acaagaccga gcagtacgtg atcaagatcc acacctttaa gcagaacaag ccccagatcc
     3721 tgaagcccat cgagaacatc cagtttatca agaccaacct gcagcagagc accctcttca
     3781 actacaccat ccacaccacc aacatcacca agaacagcat tgagctgatc tgccaggcca
     3841 tcggcctgcc caagcccacc atccagtgga tgaaggccat ctacaaccac aacaagagct
     3901 ttatcaagtg gttcaacgtg cacacctgta gcatcgacga caacaaggtg cccttcaaca
     3961 tctccatcaa ggacgaccag cagattcaga cctgcatcta ccacctgacc acactgaaag
     4021 agacactgaa ccagagccac taccagtgtc tggccaccaa tatcgccggc cacaccttca
     4081 aacagatcaa actgatctac ctggacacca ccctgatgag cagcaacgaa accatccagc
     4141 tgcagatcat gatgggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     4201 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     4261 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     4321 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     4381 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     4441 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     4501 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     4561 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     4621 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     4681 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     4741 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     4801 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     4861 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     4921 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     4981 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     5041 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     5101 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     5161 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     5221 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     5281 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     5341 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     5401 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     5461 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     5521 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     5581 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     5641 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     5701 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     5761 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     5821 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     5881 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     5941 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     6001 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     6061 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     6121 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     6181 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     6241 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     6301 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     6361 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     6421 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     6481 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     6541 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     6601 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6661 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     6721 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     6781 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     6841 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     6901 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     6961 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     7021 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     7081 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     7141 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7201 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7261 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7561 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     7621 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7681 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7741 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7801 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7861 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     7921 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7981 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8041 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8101 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8161 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8221 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8281 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8341 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8401 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8461 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8521 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8581 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8641 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8701 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8761 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8821 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8881 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8941 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9001 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9061 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9121 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9181 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9241 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9301 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9361 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     9421 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     9481 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     9541 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     9601 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
