LOCUS       sequence-229228-        9588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_018760-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4752..4769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4821..4876
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4875..4894)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7112..7130)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7198..7302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7303..7371,7372..8163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7521..7540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8334..8922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8823..8842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9076..9093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9210..9231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9246..9251,9252..9269,9270..9276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9284..9300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9289..9311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9308..9324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9308..9324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 atttcaagac ctgcgaccag agcagcttct gcacccggca gagacagatc aagcctggcg
     1261 aactggccca ctggctggaa cccagcagcg tgaagatcaa cccctacggc atgaccgccg
     1321 tgatctacac cggcgacctg aaccacagcc tgagcctgga catcacctac aaccagcaca
     1381 gcatcttcca cgtgctgatc aacgagctga ccgacgagta ccggcggttc agagtgccta
     1441 caggcgacgt gctgctgagc gtgcagaaag ctcctgtgcg gagcgtgttc gccgaggact
     1501 ctctggtgct ggatggccag tctggacaca aggccgtggt gtactacaac cccttccaca
     1561 tcgactttct gctgaacgac gtggtggtgg ccagcgccaa caagcggagc ctgctgaact
     1621 ttgagcacca gcggaagaag cccatcatca gcaagaacag cgtgtcccag aacgacaacc
     1681 acaccctgcc cagcgagaac cacgaggtgg acatcagcgc cgtgaagtcc agcgaggaca
     1741 gcagcggaaa taccggcgtg tccaaggaca gcaccaccga cgaggacaaa gagaacgaga
     1801 agcaggacca cgtgggcacc gagcactctg cccctgagga acagtcttgg agcgagtact
     1861 tcaaagagca cctggacacc agaccccacg gcctgaacag catcagcatg gacttcgatt
     1921 ttcaaggctt cagccacgtg tacggcatcc ccgagcacgc cgatggcttc gtgctgaagt
     1981 ctaccagcga cggcgacccc taccggctgt acaacctgga cgtgttcgag tacgaggtgt
     2041 acaacaagat ggccctgtac ggcagcgtgc ccgtgatgtg gggccacaac cagaacaaca
     2101 ccgtgggcgt gctgtggctg aaccccagcg aaacttgggt ggacattgac tacagcaact
     2161 ccaagaactc cggcatcttc agcggctttt ttagcaagaa gcccgagaag tcctccgtgc
     2221 agacccggtg gatgagcgag tctggcctgg tggatctgta cgtgttcatg agcagcaccc
     2281 ccagcgacgc catgagaagc tacgcctctg tcgtgggaac caccaagctg ccccctctgt
     2341 tcgccatcgg ctaccaccag tgccggtgga actacaacga cgaggccgac ctgctgtccg
     2401 tggataagca cttcgatgag tacgagatgc ctgtggatgt gctgtggctg gacatcgagc
     2461 acaccgacgg caagcggtac ttcacctggg accggaccaa gttccccaac cccaaagaaa
     2521 tggtggacaa gctgaacgtg aagggccgga agctcgtgac cgtggtggac cctcacatca
     2581 agcgggaccc caactggccc ctgttcagca acagccagaa caatggcatc ttcgtgaaaa
     2641 cccgggacgg caccgagttc gacggctggt gttggcctgg aagcagcgcc tggcccgact
     2701 tcaccgataa gtccgtgcag cagtggtggt ccaacctgtt cctgacctac gagcccgtgt
     2761 gcaaggactc catgttcacc tggaacgaca tgggcgagcc cagcgtgttc aacggccccg
     2821 aagtgaccat gcacaaggac gccaaacacg ccggcgactg ggagcacaga gacatccaca
     2881 atctgtacgg cctgtacgtg cacaagtcca cctgggatgg cctgatgctg cggagcaacg
     2941 gcgtggaaag accctttgtg ctgacccggg ccttcttcgt gggcagccag cagacagctg
     3001 ctgtgtggac cggcgacaac accgccgatt ggagccacct gaaggtgtcc acatccatgc
     3061 tgctgtctat cagcatcgtg ggcatcaccc tgtgtggcgc cgatgtgggc ggcttcttcg
     3121 gcaaccctga tagcgagctg ctgaccagat ggtatcaggc cgctgcctac cagcccttct
     3181 ttagggccca cgcccacatt gacagcaagc ggagagaacc ctggctggtg gcctccgagt
     3241 acatcgaccc catccggaag gccattcagg cccgctacca tctgctgccc tactggtaca
     3301 ccctgtttgc cagagccgag gccaacggcc agcctgtgat ggcccctatg tggttccact
     3361 tccccaagga cgtgaacacc ttcgacctgg acgatcagta catggtggga gaggccgtgc
     3421 tcgtgcggcc tgtgacagat cagggcgcct ctagcgtgca gctgtacttc cctaagggca
     3481 cctggtatca ctaccccagc ctggaagtgt ttgccggcgg acagtggatc cagtaccccg
     3541 tgaccatcaa cagcatcccc gtgttctaca gaggcggctg gatcatcccc cggaaagaga
     3601 gaatccggcg gagcagctgg ctgatgagag gcgaccctta taccttcgtg gtgttcctgg
     3661 acccccagga acctgacgcc gtgggctaca tctacatcga cgacttccac agcaccagca
     3721 agagcaacgc ccagttcttc aagatcatct accagagggt ggtggatccc accggcgctg
     3781 gcgtgcacgg cggcagactg agactgcaga gactgcctct gcccggcgag actagcatcg
     3841 tgctgcccaa ggatgatgtg ctgatcccca agatcgagcg cttcgtgatc gtgggcttca
     3901 acagccctct ggaacgcgtg acagtgatcg acgcccacaa gcccagacgg aacctgggct
     3961 ttagcgtgac ccccagctcc gtgttcagcg ccggctttaa gcacaccccc tccattgtgg
     4021 tcgtgcggaa gcccgacctg tccctgaacg atgagtggga ggtgcacttc gtgaccggca
     4081 aagagagccg ggacgacctg ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     4141 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     4201 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     4261 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     4321 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     4381 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     4441 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     4501 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     4561 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     4621 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     4681 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     4741 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     4801 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4861 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     4921 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     4981 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     5041 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     5101 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     5161 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     5221 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     5281 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     5341 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     5401 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     5461 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     5521 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     5581 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     5641 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     5701 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     5761 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     5821 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     5881 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     5941 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     6001 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     6061 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     6121 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     6181 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     6241 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     6301 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     6361 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     6421 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     6481 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     6541 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6601 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     6661 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     6721 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     6781 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     6841 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     6901 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     6961 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7021 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     7081 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7141 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7321 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7381 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7441 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7501 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7561 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7621 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7681 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7741 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7801 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     7861 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7921 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7981 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8041 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8101 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8161 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8221 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8461 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9001 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9061 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9121 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9181 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9241 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9301 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     9361 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     9421 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     9481 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     9541 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
