LOCUS       sequence-229229-        6906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_194860-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2070..2087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2139..2194
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2193..2212)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4430..4448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4516..4620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4621..4689,4690..5481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4839..4858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5652..6240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6141..6160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6394..6411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6564..6569,6570..6587,6588..6594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6602..6618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6607..6629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6626..6642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6626..6642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 aaagccaggc cagccagaaa gagctgttca ccgagagcgt gaagctgtgg aagtccatca
     1261 ccgagctgtg gaaaagattc gagcacaact gcagagtgaa gatccggaag tacctggaag
     1321 aggacaacct gggcgagaag ctggccgccg tggtgtccat ctacgtgaag cggctgaaca
     1381 agcggctgga catgagactg agcgaggacc gggccgaagg cgcgccgtcg acctccatca
     1441 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     1501 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     1561 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     1621 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     1681 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     1741 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     1801 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     1861 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     1921 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     1981 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     2041 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     2101 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     2161 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2221 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     2281 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     2341 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     2401 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     2461 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     2521 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     2581 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     2641 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     2701 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     2761 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     2821 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     2881 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     2941 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3001 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3061 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     3121 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     3181 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     3241 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     3301 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     3361 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     3421 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     3481 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     3541 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     3601 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     3661 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     3721 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     3781 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     3841 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     3901 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     3961 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4021 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4081 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4141 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4201 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4261 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4321 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     4381 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     4441 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4861 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5161 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6421 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6541 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6601 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     6661 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     6721 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     6781 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     6841 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     6901 gaccgc
//
