LOCUS       sequence-229239-        6993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_004710-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1194..1203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2157..2174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2226..2281
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2280..2299)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4517..4535)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4603..4707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4708..4776,4777..5568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4926..4945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5739..6327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6228..6247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6481..6498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6615..6636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6651..6656,6657..6674,6675..6681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6689..6705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6694..6716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6713..6729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6713..6729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tggacagcga caacgacccc agcgacatca gcgccgacaa gcggttcctg ctggccctgc
     1261 ccagccccaa gagactgagc cagcccagct acagcttcaa ccggtacaga cggcctatgt
     1321 acggctacgg ctaccggccc gactacatcg acgccgacga cgacctgttc aacgaggaca
     1381 agagatttct gctgggcctg cctcccaagg tggaacacaa acggtttctg ctcggactgc
     1441 cccccagcct gcggcagcac aagcggttta tcctgggact gcctgccccc acccggttcc
     1501 actctggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     1561 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     1621 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     1681 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     1741 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     1801 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     1861 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     1921 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     1981 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     2041 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     2101 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     2161 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     2221 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     2281 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     2341 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     2401 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     2461 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     2521 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     2581 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     2641 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     2701 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     2761 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     2821 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     2881 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     2941 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     3001 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     3061 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     3121 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     3181 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     3241 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     3301 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     3361 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     3421 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     3481 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     3541 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     3601 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     3661 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     3721 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     3781 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     3841 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     3901 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     3961 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4021 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4081 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4141 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     4201 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     4261 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4321 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     4381 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4441 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     4501 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4561 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4921 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5221 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     5281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6541 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6601 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6661 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6721 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     6781 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     6841 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     6901 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     6961 acatttatat tggctcatgt ccaatatgac cgc
//
