LOCUS       sequence-229243-        7725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_006060-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2889..2906
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2958..3013
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3012..3031)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5249..5267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5335..5439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5440..5508,5509..6300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5658..5677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6471..7059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6960..6979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7213..7230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7347..7368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7383..7388,7389..7406,7407..7413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7421..7437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7426..7448
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7445..7461
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7445..7461
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 ccaacatcta cgacagaccc ctgcccgact acatcctggc cgacgaccgg gccagccgga
     1261 tctaccacca ccggctgcag ctgtaccggc ccgagaacag cagcagcacc accaacacct
     1321 tcgtgtacat ccccaccgag ttcaacgaga acgaggccta catcagcgac ctgctgcggc
     1381 ggaatggcta cgtggccgcc atcctgagcg aggaattcgc cgacgaggcc ctgttcccca
     1441 accccacctt cagctgcacc tgtggcagag tgtccgaccc ctgcatctgc tgcggcatcc
     1501 tgcagcctca ggtgtccggc cagaaccacc tgagccccga cctgtaccag cccatcctgc
     1561 tgacccccga caataccaag cccgcccact tctgcatcaa caccacctac ctgcccaaca
     1621 agaagcagat caagatcagc gccgtgctgg tcaacctgac cagcagccag ggcgacttca
     1681 gcggcaagct ggaccccgtg atcaagaaaa gcgacaagaa cgtgatgaag ttcacctacc
     1741 tgagcaccgt gttcgatacc gtgttcctgc ccagcagccc cctgcctgcc atctgtagcg
     1801 gccggtccaa gaacaactac gtggtggacc tgtgcgtgaa cttcaccacc ctcgagtaca
     1861 agttcgacag cgacagcgac cacaagaccg tgttccacgc ctgcgccagc atccaggtgg
     1921 tgctgaaccg gatgttcgtg atcgccagat accccaagtt ctgcttcgac gcccacagag
     1981 gcgccgctgg cgacgccgat caggccagca acaacaagat gttcaacgtg aacgtgctga
     2041 tgcacaccag caaccacagc gccgactaca gcagcgtgtc cagcaagagc aagaacatca
     2101 gctacgtgga aagcagcaac cactccaaga gcagctccac cgagatcctg gacagcagct
     2161 tccaggtcgt gaacagcggc aacaaagagc ccaagagcca tctgcagaac agccacgagc
     2221 tgcaccgggg catccctggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2281 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2341 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2401 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2461 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2521 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2581 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2641 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2701 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2761 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     2821 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     2881 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     2941 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3001 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3061 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3121 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3181 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3241 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3301 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3361 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3421 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3481 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3541 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3601 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3661 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3721 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3781 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3841 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3901 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3961 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     4021 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     4081 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4141 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4201 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4261 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4321 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4381 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4441 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4501 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4561 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4621 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4681 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4741 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4801 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4861 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4921 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4981 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     5041 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     5101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5161 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5221 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5281 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5641 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7261 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7321 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7381 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7441 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7501 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7561 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     7621 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     7681 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
