LOCUS       sequence-229246-        6894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_201730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2058..2075
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2127..2182
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2181..2200)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4418..4436)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4504..4608
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4609..4677,4678..5469)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4827..4846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5640..6228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6129..6148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6382..6399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6552..6557,6558..6575,6576..6582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6595..6617
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6614..6630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6614..6630
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agttttggag agagaacaac ctgccctgca agaacctggg caccttctgc cggaagctgc
     1261 ccttcagcca gcggtgctgc gacaccaccg tgtgcgacct gaaggccccc ttcagcggca
     1321 cctgtgtgcg gtgcctggac aagggccggt tctgcctgcg gagcagagag tgctgcgata
     1381 agtactgctt cttcttcaaa tgccggggcg cgccgtcgac ctccatcacg gcctataaga
     1441 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     1501 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     1561 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     1621 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     1681 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     1741 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     1801 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     1861 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     1921 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     1981 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     2041 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     2101 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2161 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     2221 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     2281 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     2341 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     2401 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     2461 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     2521 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     2581 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     2641 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     2701 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     2761 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     2821 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     2881 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     2941 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3001 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3061 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3121 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3181 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     3241 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     3301 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     3361 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     3421 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     3481 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     3541 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     3601 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     3661 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     3721 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     3781 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     3841 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     3901 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     3961 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4021 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4081 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4141 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4201 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     4261 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4321 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     4381 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4441 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4501 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4561 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4621 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4681 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4741 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4801 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4861 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4921 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4981 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5041 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5101 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5161 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5221 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5281 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5341 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5401 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5461 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5521 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5581 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5641 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5701 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5761 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5821 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5881 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5941 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6001 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6061 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6301 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6361 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6421 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6481 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6541 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6601 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     6661 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     6721 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     6781 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     6841 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
