LOCUS       sequence-22925-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V49 pCS2+mRFP-N2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(148..166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     416..435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     610..633
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(745..764)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(746..764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgttttttnn gatctccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca
       61 aggtgcgcnt ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      181 ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc
      241 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      301 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      361 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      421 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg
      481 gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg
      541 ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga
      601 ccgacatcaa gctggacatc acctcccaca acgaggacta caccatctna aaanntnaag
      661 nnncgncnag aaggccgcca ctccaccggc gccaggatcc catcgattcg aattcaaggc
      721 ctctcgagcc tctagattct gcagccctat agtgagtcgt attacgtaga
//
