LOCUS       sequence-229258-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-MAPKAP1.1-FHH-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcca tggccttctt ggacaatcca actatcattc tagctcatat tcgacagtca
      181 catgtgacca gtgatgacac gggaatgtgt gagatggttc tcattgatca tgatgttgac
      241 ctagagaaga ttcatcctcc ttcaatgcct ggagacagtg ggtcagaaat tcagggaagc
      301 aatggtgaga ctcagggcta tgtatatgcc cagtcagtcg atattacctc aagttgggac
      361 tttggtatta gaagacgctc aaacacagct caaagattag aacgactccg aaaagagaga
      421 caaaaccaga tcaaatgcaa aaatattcag tggaaagaaa gaaattctaa gcaatcagcc
      481 caggagttaa agtcactgtt tgaaaaaaaa tctctcaaag agaagcctcc aatttctggg
      541 aagcagtcga tattatctgt acgcctagaa cagtgccctc tgcagctgaa taaccctttt
      601 aacgagtatt ccaaatttga tggcaagggt catgtaggta caacagcaac caagaagatc
      661 gatgtctacc tccctctgca ctcgagccag gacagactgc tgccaatgac cgtggtgaca
      721 atggccagcg ccagggtgca ggacctgatc gggctcatct gctggcagta tacaagcgaa
      781 ggacgggagc cgaagctcaa tgacaatgtc agtgcctact gcctgcatat tgctgaggat
      841 gatggggagg tggacaccga tttccccccg ctggattcca atgagccc
//
