LOCUS       sequence-229259-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-MAPKAP1.1-FHH-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             666..689
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             696..722
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             732..749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaatcttact gaaggcagtg aagcgaagaa aaggatccca gaaagtttca ggccctcagt
       61 accgcctgga gaagcagagc gagcccaatg tcgccgttga cctggacagc actttggaga
      121 gccagagcgc atgggagttc tgcctggtcc gcgagaacag ttcaagggca gacggggttt
      181 ttgaggagga ttcgcaaatt gacatagcca cagtacagga tatgcttagc agccaccatt
      241 acaagtcatt caaagtcagc atgatccaca gactgcgatt cacaaccgac gtacagctag
      301 gtatctctgg agacaaagta gagatagacc ctgttacgaa tcagaaagcc agcactaagt
      361 tttggattaa gcagaaaccc atctcaatcg attccgacct gctctgtgcc tgtgaccttg
      421 ctgaagagaa aagccccagt cacgcaatat ttaaactcac gtatctaagc aatcacgact
      481 ataaacacct ctactttgaa tcggacgctg ctaccgtcaa tgaaattgtg ctcaaggtta
      541 actacatcct ggaatcgcga gctagcactg cccgggctga ctactttgct caaaaacaaa
      601 gaaaactgaa cagacgtacg agcttcagct tccagaagga gaagaaatcc gggcagcagc
      661 tcgaggatta taaagatgac gacgataaag caggttaccc atacgatgtg cctgactatg
      721 ctgcaggttc acatcatcac caccatcatt gagtcgagtc tagagggccc gacgcgtctg
      781 gccaccg
//
