LOCUS       sequence-22926-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: M78 pIRESpuro-CBP-2xTEV-StrepTag
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..129
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             157..177
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             193..213
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             238..351
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     intron          422..651
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     634..653
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gatatctgcg gccgcgtcga cggaattcgc ccttttcggc gcgcctgtac aaagcggcgg
       61 tggaagaaga acttcatcgc cgtgagcgcc gccaaccggt tcaagaagat cagcagcagc
      121 ggcgccctga tcgatggcgg cagcggcccc ggcggcgaga acctgtactt ccagggcgcc
      181 atccccacca ccgagaacct gtacttccag agcggcggcg gcagcggccc cggcggcatg
      241 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
      301 gagcagctgc gggcccggct ggagcaccac ccccagggcc agcgggagcc ctagggatcc
      361 actagtaacg gccgccagtg tgctggaatt aattcgctgt ctgcgagggc cagctgttgg
      421 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
      481 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
      541 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
      601 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtcca
//
