LOCUS       sequence-229266-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-MAPKAP1Del-FHH-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             713..736
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             743..769
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             779..796
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctac
      121 tagaggatcc atggccttct tggacaatcc aactatcatt ctagctcata ttcgacagtc
      181 acatgtgacc agtgatgaca cgggaatgtg tgagatggtt ctcattgatc atgatgttga
      241 cctagagaag attcatcctc cttcaatgcc tggagacagt gggtcagaaa ttcagggaag
      301 caatggtgag actcagggct atgtatatgc ccagtcagtc gatattacct caagttggga
      361 ctttggtatt agaagacgct caaacacagc tcaaagatta gaacgactcc gaaaagagag
      421 acaaaaccag atcaaatgca aaaatattca gtggaaagaa agaaattcta agcaatcagc
      481 ccaggagtta aagtcactgt ttgaaaaaaa atctctcaaa gagaagcctc caatttctgg
      541 gaagcagtcg atattatctg tacgcctaga acagtgccct ctgcagctga ataacccttt
      601 taacgagtat tccaaatttg atggcaaggg tcatgtaggt acaacagcaa ccaagaagat
      661 cgatgtctac ctccctctgc actcgagcca ggacagactg ctgccactcg aggattataa
      721 agatgacgac gataaagcag gttacccata cgatgtgcct gactatgctg caggttcaca
      781 tcatcaccac catcattgag tcgagtctag agggcccgac gcgtctggcc accggtcgcc
      841 cctctccctc ccccccccct aacgttac
//
