LOCUS       sequence-229267-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-MAPKAP1Del-FHH-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..51
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             698..721
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             728..754
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             764..781
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
       61 cgccatccac gctgttttga cctccataga agacaccgac tctactagag gatccatggc
      121 cttcttggac aatccaacta tcattctagc tcatattcga cagtcacatg tgaccagtga
      181 tgacacggga atgtgtgaga tggttctcat tgatcatgat gttgacctag agaagattca
      241 tcctccttca atgcctggag acagtgggtc agaaattcag ggaagcaatg gtgagactca
      301 gggctatgta tatgcccagt cagtcgatat tacctcaagt tgggactttg gtattagaag
      361 acgctcaaac acagctcaaa gattagaacg actccgaaaa gagagacaaa accagatcaa
      421 atgcaaaaat attcagtgga aagaaagaaa ttctaagcaa tcagcccagg agttaaagtc
      481 actgtttgaa aaaaaatctc tcaaagagaa gcctccaatt tctgggaagc agtcgatatt
      541 atctgtacgc ctagaacagt gccctctgca gctgaataac ccttttaacg agtattccaa
      601 atttgatggc aagggtcatg taggtacaac agcaaccaag aagatcgatg tctacctccc
      661 tctgcactcg agccaggaca gactgctgcc actcgaggat tataaagatg acgacgataa
      721 agcaggttac ccatacgatg tgcctgacta tgctgcaggt tcacatcatc accaccatca
      781 ttgagtcgag tctagagggc ccgacgcgtc tggccaccgg tcg
//
