LOCUS       sequence-22927-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V48 pCS2+mRFP-N1
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..687)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(13..687)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(72..95)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(270..289)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     539..557
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(722..740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(723..740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgatgggat ccggcgccgg tggagtggcg gccctcggcg cgctcgtact gttccacgat
       61 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg tcggtcttgt aggcgccggg
      121 cagctgcacg ggcttcttgg ccatgtaggt ggtcttgacc tcggcgtcgt agtggccgcc
      181 gtccttcagc ttcagcctca tcttgatctc gcccttcagg gcgccgtcct cggggtacat
      241 ccgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
      301 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
      361 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      421 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      481 ggagccgtac tggaactgag gggacaggat gtcccaggcg aagggcaggg ggccgccctt
      541 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
      601 gatctcgaac tcgtggccgt tcacggagcc ctccatgcgc accttgaagc gcatgaactc
      661 cttgatgacg tcctcggagg aggccatggt agatcctgca aaaagaacaa gtagcttgta
      721 ttctatagtg tcacctaaat caagcttgct ccgacgtccc caggcagaat ggcggttccc
      781 taaacgagca ttgcttatat agacctccca ttaggcacgc ctaccgccca tttacgtcaa
      841 tggaacgccc atttgcgtca ttgcccctcc ccattgacgt cnntggggat gtacttggca
      901 gccatcgcgg gccatttacc gccattg
//
